Synthesis and Application of D- and 13C-Labelled tert-Butyl Hoechst Dye

IF 0.9 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of labelled compounds & radiopharmaceuticals Pub Date : 2024-10-23 DOI:10.1002/jlcr.4123

Yulia A. Vlasenko, Avery J. To, Tess Fortier, Natasha M. Evans, Cole J. Lindsay, Peter J. Palermo, Thorsten Dieckmann, Graham K. Murphy

引用次数: 0

Abstract

Herein, the successful syntheses of D₃- and ¹³C-N-methyl and D₉-tert-butyl Hoechst dyes are presented. This includes the preparation of the labelled D₃- and ¹³C-N-methyl piperazines and D₉-tert-butylated hydroxytoluene precursors. The tert-butyl Hoechst dye is known to bind a specific RNA aptamer. Spectroscopic NMR studies of the labelled Hoechst dye-aptamer complexes allowed for the unambiguous assignment of chemical shifts, as well as the dynamics of the bound dye.

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D- 和 13C 标记叔丁基 Hoechst 染料的合成与应用。

本文介绍了 D3-、13C-N-甲基和 D9-叔丁基 Hoechst 染料的成功合成。这包括制备标记的 D3-和 13C-N-methyl 哌嗪和 D9-tert-butylated hydroxytoluene 前体。已知叔丁基 Hoechst 染料能与特定的 RNA 合体结合。通过对标记的 Hoechst 染料-适配体复合物进行光谱核磁共振研究，可以明确地确定化学位移以及结合染料的动态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of labelled compounds & radiopharmaceuticals 医学-分析化学

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Labelled Compounds and Radiopharmaceuticals publishes all aspects of research dealing with labeled compound preparation and applications of these compounds. This includes tracer methods used in medical, pharmacological, biological, biochemical and chemical research in vitro and in vivo. The Journal of Labelled Compounds and Radiopharmaceuticals devotes particular attention to biomedical research, diagnostic and therapeutic applications of radiopharmaceuticals, covering all stages of development from basic metabolic research and technological development to preclinical and clinical studies based on physically and chemically well characterized molecular structures, coordination compounds and nano-particles.