{"title":"Synchrotron X-ray imaging of soft biological tissues - principles, applications and future prospects.","authors":"Jonas Albers, Angelika Svetlove, Elizabeth Duke","doi":"10.1242/jcs.261953","DOIUrl":null,"url":null,"abstract":"<p><p>Synchrotron-based tomographic phase-contrast X-ray imaging (SRµCT or SRnCT) is a versatile isotropic three-dimensional imaging technique that can be used to study biological samples spanning from single cells to human-sized specimens. SRµCT and SRnCT take advantage of the highly brilliant and coherent X-rays produced by a synchrotron light source. This enables fast data acquisition and enhanced image contrast for soft biological samples owing to the exploitation of phase contrast. In this Review, we provide an overview of the basics behind the technique, discuss its applications for biologists and provide an outlook on the future of this emerging technique for biology. We introduce the latest advances in the field, such as whole human organs imaged with micron resolution, using X-rays as a tool for virtual histology and resolving neuronal connections in the brain.</p>","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"137 20","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529875/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of cell science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1242/jcs.261953","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/23 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Synchrotron-based tomographic phase-contrast X-ray imaging (SRµCT or SRnCT) is a versatile isotropic three-dimensional imaging technique that can be used to study biological samples spanning from single cells to human-sized specimens. SRµCT and SRnCT take advantage of the highly brilliant and coherent X-rays produced by a synchrotron light source. This enables fast data acquisition and enhanced image contrast for soft biological samples owing to the exploitation of phase contrast. In this Review, we provide an overview of the basics behind the technique, discuss its applications for biologists and provide an outlook on the future of this emerging technique for biology. We introduce the latest advances in the field, such as whole human organs imaged with micron resolution, using X-rays as a tool for virtual histology and resolving neuronal connections in the brain.
基于同步加速器的断层相位对比 X 射线成像(SRµCT 或 SRnCT)是一种通用的各向同性三维成像技术,可用于研究从单细胞到人体大小的生物样本。SRµCT 和 SRnCT 利用同步辐射光源产生的高亮度和相干 X 射线。由于利用了相位对比,因此可以快速获取数据,并增强软性生物样本的图像对比度。在这篇综述中,我们将概述这项技术背后的基本原理,讨论它在生物学家中的应用,并展望这项新兴生物技术的未来。我们将介绍该领域的最新进展,如以微米分辨率成像人体器官、使用 X 射线作为虚拟组织学工具以及解析大脑神经元连接等。