Understanding metabolic plasticity at single cell resolution.

IF 5.6 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Essays in biochemistry Pub Date : 2024-11-18 DOI:10.1042/EBC20240002

Christina C Abbate, Jason Hu, John G Albeck

引用次数: 0

Abstract

It is increasingly clear that cellular metabolic function varies not just between cells of different tissues, but also within tissues and cell types. In this essay, we envision how differences in central carbon metabolism arise from multiple sources, including the cell cycle, circadian rhythms, intrinsic metabolic cycles, and others. We also discuss and compare methods that enable such variation to be detected, including single-cell metabolomics and RNA-sequencing. We pay particular attention to biosensors for AMPK and central carbon metabolites, which when used in combination with metabolic perturbations, provide clear evidence of cellular variance in metabolic function.

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以单细胞分辨率了解代谢可塑性。

人们越来越清楚地认识到，细胞代谢功能不仅在不同组织细胞之间存在差异，而且在组织和细胞类型内部也存在差异。在这篇文章中，我们设想了中心碳代谢的差异是如何从多种来源产生的，包括细胞周期、昼夜节律、内在代谢周期等。我们还讨论并比较了能够检测这种差异的方法，包括单细胞代谢组学和 RNA 测序。我们特别关注 AMPK 和中心碳代谢物的生物传感器，当它们与代谢扰动结合使用时，可提供细胞代谢功能差异的明确证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Essays in biochemistry 生物-生化与分子生物学

CiteScore

10.50

自引率

0.00%

发文量

105

审稿时长

>12 weeks

期刊介绍： Essays in Biochemistry publishes short, digestible reviews from experts highlighting recent key topics in biochemistry and the molecular biosciences. Written to be accessible for those not yet immersed in the subject, each article is an up-to-date, self-contained summary of the topic. Bridging the gap between the latest research and established textbooks, Essays in Biochemistry will tell you what you need to know to begin exploring the field, as each article includes the top take-home messages as summary points. Each issue of the journal is guest edited by a key opinion leader in the area, and whether you are continuing your studies or moving into a new research area, the Journal gives a complete picture in one place. Essays in Biochemistry is proud to publish Understanding Biochemistry, an essential online resource for post-16 students, teachers and undergraduates. Providing up-to-date overviews of key concepts in biochemistry and the molecular biosciences, the Understanding Biochemistry issues of Essays in Biochemistry are published annually in October.