Cytotoxic activity, selectivity, and clonogenicity of fruits and resins of Saudi medicinal plants against human liver adenocarcinoma.

IF 2 Q3 PHARMACOLOGY & PHARMACY
Drug Target Insights Pub Date : 2024-10-22 eCollection Date: 2024-01-01 DOI:10.33393/dti.2024.3169
Ali Hendi Alghamdi, Aimun A E Ahmed, Mahadi Bashir, Haidar Abdalgadir, Asaad Khalid, Mohamed E Abdallah, Riyad Almaimani, Bassem Refaat, Ashraf N Abdalla
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引用次数: 0

Abstract

Background: Edible fruits and resins provide various benefits to mankind including potential medicinal applications. This study aimed to determine the cytotoxicity, selectivity, and clonogenicity of fruits and exudates of certain Saudi medicinal plants (Anethum graveolens (BEP-09), Opuntia ficus-indica (L.) Miller (BEP-10), Boswellia serrata Roxb. ex Colebr. (BEP-11), and Commiphora myrrha (BEP-12)) against human liver adenocarcinoma (HepG2).

Methods: Initial cytotoxicity and cell line selectivity against different cell lines were screened using MTT assay. The most promising extract was subjected to gas chromatography-mass spectrometry (GC-MS) analysis to determine the main phytoconstituents. Clonogenicity was checked for the most active extract.

Results: The selected plants' fruits and resins possess a significant cytotoxic activity estimated as IC50. The fruit of BEP-10 was found to be the most active extract against liver cancer cells (IC50 = 2.82) comparable to both doxorubicin (IC50 = 1.40) and camptothecin (IC50 = 1.11). It showed a selectivity index of 4.47 compared to the normal human foetal lung fibroblast (MRC5) cells. BEP-10 showed a dose-dependent clonogenic effect against HepG2 cells comparable to the effect of doxorubicin. The GC-MS chromatogram of BEP-10 extract revealed the presence of eight small polar molecules, representing 73% of the total identified compounds and the rest three molecules (27%) were non-polar constituents. The furan derivatives represent the chief components in BEP-10 (16.3%), while the aldehyde 5-(hydroxymethyl)-2-furancarboxaldehyde was found to be the main molecule (13.2%).

Conclusion: The fruits of BEP-10 have a potential cytotoxic effect particularly against HepG2. The identified phytoconstituents in the tested plant extract might contribute to the investigated cytotoxic activity.

沙特药用植物果实和树脂对人类肝腺癌的细胞毒活性、选择性和克隆性。
背景:食用水果和树脂为人类带来了各种益处,包括潜在的药用价值。本研究旨在确定某些沙特药用植物(Anethum graveolens (BEP-09)、Opuntia ficus-indica (L.) Miller (BEP-10)、Boswellia serrata Roxb. ex Colebr. (BEP-11) 和 Commiphora myrrha (BEP-12))的果实和渗出物对人肝癌(HepG2)的细胞毒性、选择性和克隆性:方法:使用 MTT 法筛选对不同细胞系的初始细胞毒性和细胞系选择性。对最有希望的提取物进行气相色谱-质谱(GC-MS)分析,以确定其主要植物成分。对最具活性的提取物进行了克隆生成性检测:结果:所选植物的果实和树脂具有显著的细胞毒性活性(IC50)。发现 BEP-10 果实对肝癌细胞的活性最高(IC50 = 2.82),与多柔比星(IC50 = 1.40)和喜树碱(IC50 = 1.11)相当。与正常人胎肺成纤维细胞(MRC5)相比,它的选择性指数为 4.47。BEP-10 对 HepG2 细胞具有与多柔比星相当的剂量依赖性克隆生成效应。BEP-10 提取物的气相色谱-质谱(GC-MS)色谱图显示存在 8 种极性小分子,占已鉴定化合物总量的 73%,其余 3 种分子(27%)为非极性成分。呋喃衍生物是 BEP-10 的主要成分(16.3%),而醛 5-(羟甲基)-2-呋喃甲醛则是主要分子(13.2%):结论:BEP-10 果实具有潜在的细胞毒性作用,特别是对 HepG2。结论:BEP-10 果实具有潜在的细胞毒性作用,特别是对 HepG2。
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来源期刊
Drug Target Insights
Drug Target Insights PHARMACOLOGY & PHARMACY-
CiteScore
2.70
自引率
0.00%
发文量
5
审稿时长
8 weeks
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