Microgravity as a Tool to Investigate Cancer Induction in Pleura Mesothelial Cells.

IF 2.8 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Issues in Molecular Biology Pub Date : 2024-09-27 DOI:10.3390/cimb46100647

Valentina Bonetto, Corinna Anais Pagano, Maurizio Sabbatini, Valeria Magnelli, Massimo Donadelli, Emilio Marengo, Maria Angela Masini

{"title":"Microgravity as a Tool to Investigate Cancer Induction in Pleura Mesothelial Cells.","authors":"Valentina Bonetto, Corinna Anais Pagano, Maurizio Sabbatini, Valeria Magnelli, Massimo Donadelli, Emilio Marengo, Maria Angela Masini","doi":"10.3390/cimb46100647","DOIUrl":null,"url":null,"abstract":"<p><p>The present work shows that the exposure of mesothelial cells to simulated microgravity changes their cytoskeleton and adhesion proteins, leading to a cell switch from normal towards tumoral cells. Immunohistochemical and molecular data were obtained from both MeT-5A exposed to simulated microgravity and BR95 mesothelioma cell lines. Simulated microgravity was found to affect the expression of actin, vinculin, and connexin-43, altering their quantitative and spatial distribution pattern inside the cell. The analysis of the tumoral markers p27, CD44, Fibulin-3, and NANOG and the expression of genes related to cancer transformation such as NANOG, CDH-1, and Zeb-1 showed that the simulated microgravity environment led to expression patterns in MeT-5A cells similar to those observed in BR95 cells. The alteration in both quantitative expression and structural organization of the cytoskeleton and adhesion/communication proteins can thus be considered a pivotal mechanism involved in the cellular shift towards tumoral progression.</p>","PeriodicalId":10839,"journal":{"name":"Current Issues in Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506709/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Issues in Molecular Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/cimb46100647","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The present work shows that the exposure of mesothelial cells to simulated microgravity changes their cytoskeleton and adhesion proteins, leading to a cell switch from normal towards tumoral cells. Immunohistochemical and molecular data were obtained from both MeT-5A exposed to simulated microgravity and BR95 mesothelioma cell lines. Simulated microgravity was found to affect the expression of actin, vinculin, and connexin-43, altering their quantitative and spatial distribution pattern inside the cell. The analysis of the tumoral markers p27, CD44, Fibulin-3, and NANOG and the expression of genes related to cancer transformation such as NANOG, CDH-1, and Zeb-1 showed that the simulated microgravity environment led to expression patterns in MeT-5A cells similar to those observed in BR95 cells. The alteration in both quantitative expression and structural organization of the cytoskeleton and adhesion/communication proteins can thus be considered a pivotal mechanism involved in the cellular shift towards tumoral progression.

查看原文本刊更多论文

将微重力作为研究胸膜间皮细胞癌症诱导的工具

本研究表明，间皮细胞暴露在模拟微重力环境中会改变其细胞骨架和粘附蛋白，导致细胞从正常细胞向肿瘤细胞转变。研究人员从暴露于模拟微重力的 MeT-5A 和 BR95 间皮瘤细胞系中获得了免疫组化和分子数据。研究发现，模拟微重力会影响肌动蛋白、长春新碱和连接蛋白-43的表达，改变它们在细胞内的数量和空间分布模式。对肿瘤标志物p27、CD44、Fibulin-3和NANOG以及与癌症转化相关的基因（如NANOG、CDH-1和Zeb-1）的表达进行的分析表明，模拟微重力环境导致MeT-5A细胞的表达模式与在BR95细胞中观察到的类似。因此，细胞骨架和粘附/通讯蛋白的定量表达和结构组织的改变可被视为细胞向肿瘤进展转变的关键机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current Issues in Molecular Biology 生物-生化研究方法

CiteScore

2.90

自引率

3.20%

发文量

380

审稿时长

>12 weeks

期刊介绍： Current Issues in Molecular Biology (CIMB) is a peer-reviewed journal publishing review articles and minireviews in all areas of molecular biology and microbiology. Submitted articles are subject to an Article Processing Charge (APC) and are open access immediately upon publication. All manuscripts undergo a peer-review process.