Population genetic differentiation and structure of rare plant Anemone shikokiana based on genotyping-by-sequencing (GBS).

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-10-23 DOI:10.1186/s12870-024-05705-1

Kang An, Chunxia Zhou, Boqiang Tong, Dan Liu, Xiaohan Shan, Xin Zhang, Fuhua Bian

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引用次数: 0

Abstract

Background: Anemone shikokiana (Makino) Makino is a perennial herb of the genus Anemone in the family Ranunculaceae. Endemic to the Shandong Peninsula in China and Shikoku Island in Japan, it is a rare and endangered plant with a narrow, disjunct distribution. It is threatened with extinction and is in urgent need of conservation. Evaluating the genetic diversity of species, revealing the population genetic structure and gene flow, and inferring the population history are of great importance for species conservation, especially for rare and endangered plants.

Results: In our study, 73 samples from eight wild populations in China were sequenced by Super-GBS, yielding a total of 40.59 G clean reads and 52,231 SNPs. Based on the obtained SNP data set, we evaluated the population genetic diversity, genetic structure, and gene flow of A. shikokiana. A low level of genetic diversity was found (He = 0.1925, Ho = 0.1422). The neighbor-joining (NJ) tree, principal component analysis and ADMIXTURE analysis suggested that these 73 A. shikokiana could be considered as two groups. Pairwise genetic differentiation coefficients (Fst) indicated that genetic differentiation was lower between adjacent populations and higher between geographically separated populations. The gene flow between Kunyu Mountain and Lao Mountain was very low. However, neither of the two regions showed evidence of Isolation by Distance.

Conclusions: Here, we revealed the population genetic structure and gene flow of A. shikokiana from the Shandong Peninsula, China. This research provides valuable genetic resources for A. shikokiana and contributes to the scientific and effective conservation of the species.

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基于基因分型测序（GBS）的珍稀植物石莲花（Anemone shikokiana）种群遗传分化和结构。

背景介绍牧野海葵（Anemone shikokiana (Makino) Makino）是毛茛科海葵属多年生草本植物。它是中国山东半岛和日本四国岛特有的珍稀濒危植物，分布范围狭窄且互不相连。它濒临灭绝，急需保护。评估物种遗传多样性、揭示种群遗传结构和基因流、推断种群历史对物种保护，尤其是珍稀濒危植物的保护具有重要意义：在我们的研究中，Super-GBS 对来自中国 8 个野生种群的 73 个样本进行了测序，共获得 40.59 G 的纯净读数和 52,231 个 SNPs。根据获得的 SNP 数据集，我们评估了石蒜的种群遗传多样性、遗传结构和基因流。结果发现遗传多样性水平较低（He = 0.1925，Ho = 0.1422）。邻接（NJ）树、主成分分析和 ADMIXTURE 分析表明，这 73 个 A. shikokiana 可被视为两个群体。成对遗传分化系数（Fst）表明，相邻种群之间的遗传分化程度较低，而地理上相隔较远的种群之间的遗传分化程度较高。昆玉山和老山之间的基因流动非常低。然而，这两个地区都没有出现 "距离隔离"（Isolation by Distance）的迹象：本文揭示了中国山东半岛 A. shikokiana 的种群遗传结构和基因流。该研究为 A. shikokiana 提供了宝贵的遗传资源，有助于科学、有效地保护该物种。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.