Aggregation induced emission "Turn on" ultra-low detection of anti-inflammatory drug flufenamic acid in human urine samples by carbon dots derived from bamboo stem waste.

Subitha Adaikalapandi, T Daniel Thangadurai, S Sivakumar, D Nataraj, Alex Schechter, Nandakumar Kalarikkal, Sabu Thomas
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Abstract

Carbon dot-based fluorescence sensors have attracted research interest for the selective determination of anti-inflammatory drugs in biological fluids and environments. The overdose and accumulation of anti-inflammatory drugs in tissues can cause chronic side effects including abdominal pain, and renal damage. Herein, we report a new fluorescent probe, bamboo stem waste-derived carbon dots (BS-CDs) for highly sensitive detection of Flufenamic acid (FA), a hazardous anti-inflammatory drug. The UV-vis absorption spectra of BS-CDs show a redshifted absorption peak at 283 nm upon the addition of FA suggesting strong binding interaction between BS-CDs and FA molecule. The BS-CDs showed a fluorescence enhancement (∼2-fold) detection for FA (400 μM) in the linear concentration range (0.40 → 0.65 μM) with a limit of detection (LoD; 17 nM) and binding constant (Ka = 1.33 × 10-3 M-1). The time-resolved fluorescence decay analysis showed that the average lifetime of BS-CDs has slightly changed (4.42 → 4.67 ns) by the interaction with FA through the aggregation-induced emission (AIE) process. The interference, pH, and effect of time results suggest that BS-CDs are highly selective probes for FA detection and do not show any interference involvement during FA detection. The confirmation of the structure and morphology changes of BS-CDs after interaction with FA was carried out by XRD, FESEM, HRTEM, FTIR, and Raman spectroscopy. The practicability of the BS-CDs probe was proved by the detection of FA in human urine samples with recovery of 103-109 %. This suggests that the proposed BS-CDs-based 'turn-on' sensor could be used to determine the FA in biological fluids.

利用竹茎废料提取的碳点聚合诱导发射 "开启 "超低检测人体尿样中的消炎药氟芬那酸。
基于碳点的荧光传感器在生物液体和环境中选择性测定消炎药方面引起了研究兴趣。过量服用消炎药并在组织中蓄积会导致慢性副作用,包括腹痛和肾损伤。在此,我们报告了一种新型荧光探针--竹茎废弃物衍生碳点(BS-CDs),用于高灵敏度检测有害消炎药氟芬那酸(FA)。加入氟芬那酸后,BS-CDs 的紫外-可见吸收光谱在 283 纳米处出现红移吸收峰,表明 BS-CDs 与氟芬那酸分子之间有很强的结合作用。在线性浓度范围(0.40 → 0.65 μM)内,BS-CDs 对 FA(400 μM)的检测荧光增强(∼2 倍),检测限(LoD;17 nM)和结合常数(Ka = 1.33 × 10-3 M-1)。时间分辨荧光衰减分析表明,通过聚集诱导发射(AIE)过程与 FA 相互作用,BS-CD 的平均寿命略有改变(4.42 → 4.67 ns)。干扰、pH 值和时间的影响结果表明,BS-CDs 是一种高选择性的 FA 检测探针,在 FA 检测过程中不会受到任何干扰。XRD、FESEM、HRTEM、傅立叶变换红外光谱和拉曼光谱证实了 BS-CD 与 FA 作用后的结构和形态变化。BS-CDs 探针在人体尿液样本中检测到了 FA,回收率为 103-109%,这证明了 BS-CDs 探针的实用性。这表明所提出的基于 BS-CDs 的 "开启式 "传感器可用于测定生物液体中的 FA。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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