TiRNA-Gly-GCC-002 is associated with progression in patients with hepatocellular carcinoma.

IF 1.5 4区医学 Q4 ONCOLOGY

Translational cancer research Pub Date : 2024-09-30 Epub Date: 2024-09-27 DOI:10.21037/tcr-24-644

Lili Wu, Lijiang Zhang, Jie Cao, Yunpeng Sun, Jiajia Zhang, Liang Shi, Yong Xia

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引用次数: 0

Abstract

Background: The transfer RNA (tRNA)-derived fragments, generated by the cleavage of mature and pre-tRNAs, play a vital role in the tumorigenesis and progression of hepatocellular carcinoma (HCC). However, the relationship between tRNA-derived fragments and the prognosis of patients with HCC has not been thoroughly studied. This study aims to discuss the relationship between tiRNA-Gly-GCC-002 and the prognosis of HCC patients and its role in guiding HCC treatment.

Methods: In this study, the differently expressed tRNA-derived fragments were screened out from the tumor tissues and paracancerous tissues. These tRNA-derived fragments were validated in the tissues and serum samples of patients with HCC by quantitative real-time polymerase chain reaction (qRT-PCR). The target genes of the tRNA-derived fragments were predicted with the microRNA target prediction database (miRDB), which was proceeded with gene set enrichment analysis (GSEA). After that, we analyzed the prognostic effect of the tRNA-derived fragment in relapse-free survival (RFS). Based on univariate and multivariate Cox regression analysis, independent prognostic factors for RFS were obtained. In addition, a column chart was constructed based on clinical pathological features and tiRNAGly-GCC-002.

Results: The tiRNA-Gly-GCC-002 was ultimately served as the candidate gene. Function analysis indicated that tiRNA-Gly-GCC-002 was primarily involved in adenyl nucleotide binding, cell cycle, cell cycle process and chromosome organization. We found that patients with high expression level of tiRNA-Gly-GCC-002 had worse prognosis than low expression level. The univariable and multivariable Cox regression analyses showed that tiRNAGly-GCC-002 was an important prognostic factor. Furthermore, the nomogram by combining tiRNA-Gly-GCC-002 expression level (P=0.03) and serum gamma-glutamyl transferase (GGT) level (P=0.001) was established to predict the prognosis of patients with HCC [concordance index (C-index): 0.789].

Conclusions: In summary, the tiRNA-Gly-GCC-002 can predict the outcome of patients with HCC, which may play a vital role in directing the treatment of HCC.

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TiRNA-Gly-GCC-002 与肝细胞癌患者的病情进展有关。

背景：由成熟和前tRNA裂解产生的转移RNA（tRNA）衍生片段在肝细胞癌（HCC）的肿瘤发生和发展过程中起着至关重要的作用。然而，tRNA 衍生片段与 HCC 患者预后之间的关系尚未得到深入研究。本研究旨在探讨tiRNA-Gly-GCC-002与HCC患者预后的关系及其在指导HCC治疗中的作用：方法：本研究从肿瘤组织和癌旁组织中筛选出不同表达的 tRNA 衍生片段。方法：本研究从肿瘤组织和癌旁组织中筛选出了不同表达的 tRNA 衍生片段，并通过实时定量聚合酶链反应（qRT-PCR）在 HCC 患者的组织和血清样本中验证了这些 tRNA 衍生片段。利用 microRNA 靶点预测数据库（miRDB）预测了 tRNA 衍生片段的靶基因，并进行了基因组富集分析（GSEA）。之后，我们分析了tRNA衍生片段对无复发生存率（RFS）的预后影响。根据单变量和多变量 Cox 回归分析，得出了 RFS 的独立预后因素。此外，还根据临床病理特征和tiRNAGly-GCC-002构建了柱状图：结果：tiRNA-Gly-GCC-002最终成为候选基因。功能分析表明，tiRNA-Gly-GCC-002 主要参与腺嘌呤核苷酸结合、细胞周期、细胞周期过程和染色体组织。我们发现，tiRNA-Gly-GCC-002表达水平高的患者预后比表达水平低的患者差。单变量和多变量 Cox 回归分析表明，tiRNAGly-GCC-002 是一个重要的预后因素。此外，通过结合 tiRNA-Gly-GCC-002 表达水平（P=0.03）和血清γ-谷氨酰转移酶（GGT）水平（P=0.001），建立了预测 HCC 患者预后的提名图[一致性指数（C-index）：0.789]：综上所述，tiRNA-Gly-GCC-002 可预测 HCC 患者的预后，这可能对指导 HCC 的治疗起到重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational cancer research ONCOLOGY-

CiteScore

2.10

自引率

0.00%

发文量

252

期刊介绍： Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.