Preoperative abaloparatide plus zoledronate treatment accelerates femoral bone healing in rats following osteotomy.

IF 1.6 4区 医学
Takashi Kataoka, Yuta Tsubouchi, Ryota Takase, Takefumi Otsu, Tatsuya Iwasaki, Masashi Kataoka, Nobuhiro Kaku
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引用次数: 0

Abstract

Purpose: We investigated the potential efficacy of abaloparatide (Abalo) and zoledronate (ZA) combination therapy for accelerating femoral union in a rat osteotomy model. Methods: Nine-week-old male Sprague-Dawley rats were randomly divided into four groups (n = 14 per group): control, abaloparatide (Abalo, 30 μg/kg via subcutaneous injection [s.c.] 5 times per week for 6 weeks), zoledronate (ZA; 0.1 mg/kg via s.c., single dose), and Abalo + ZA. Rats were then subjected to unilateral osteotomy of the femoral shaft, followed by osteosynthesis with intramedullary nailing to establish bone healing models. At 2 and 4 weeks after osteotomy, both femurs were removed from seven rats per group for soft X-ray imaging to evaluate bone union and microcomputed tomography (micro-CT) for bone morphometric evaluation. Blood samples were collected from all rats every 2 week starting 6 weeks pre- to 4 weeks postosteotomy. Toluidine blue staining was used for histopathological evaluation of the undecalcified specimens. Results: Soft X-ray imaging revealed accelerated callus formation, callus maturation, and fracture line closure in Abalo and Abalo + ZA groups compared to ZA and control groups. Micro-CT demonstrated greater cortical bone and trabecular bone to total volume ratios in contralateral (left) femurs of the Abalo + ZA group compared to the Abalo group. Both trabecular and cortical bone mineral densities were also greater in contralateral femurs of the Abalo + ZA group compared to Abalo and ZA groups. Conclusion: These findings suggest substantial additive or synergistic efficacy of abaloparatide plus zoledronate combination therapy for accelerating bone healing following osteotomy and for maintaining normal bone health.

术前阿巴拉帕肽加唑来膦酸钠治疗可加速大鼠截骨后的股骨头愈合。
目的:我们研究了阿巴拉帕肽(Abaloparatide,Abalo)和唑来膦酸钠(zoledronate,ZA)联合疗法在大鼠截骨模型中加速股骨结合的潜在疗效。研究方法将九周大的雄性 Sprague-Dawley 大鼠随机分为四组(每组 n = 14):对照组、阿巴拉帕肽(Abaloparatide,30 μg/kg 经皮下注射 [s.c.],每周 5 次,共 6 周)组、唑来膦酸钠(ZA,0.1 mg/kg 经皮下注射,单剂量)组和阿巴拉帕肽 + ZA 组。然后对大鼠进行单侧股骨干截骨,再用髓内钉进行骨合成,以建立骨愈合模型。截骨后 2 周和 4 周,每组取出 7 只大鼠的股骨,进行软 X 射线成像以评估骨结合情况,并进行微计算机断层扫描(micro-CT)以评估骨形态。从截骨前 6 周到截骨后 4 周,每 2 周采集一次所有大鼠的血液样本。甲苯胺蓝染色用于对未钙化标本进行组织病理学评估。结果软X射线成像显示,与ZA组和对照组相比,Abalo组和Abalo + ZA组的胼胝形成、胼胝成熟和骨折线闭合速度更快。显微 CT 显示,与 Abalo 组相比,Abalo + ZA 组对侧(左侧)股骨的皮质骨和骨小梁占总体积的比例更大。与阿巴洛组和ZA组相比,阿巴洛+ZA组对侧股骨的骨小梁和皮质骨矿物质密度也更高。结论:这些研究结果表明,阿巴拉帕肽+唑来膦酸钠联合疗法在加速截骨术后骨愈合和维持正常骨骼健康方面具有显著的相加或协同疗效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
自引率
0.00%
发文量
91
期刊介绍: Journal of Orthopaedic Surgery is an open access peer-reviewed journal publishing original reviews and research articles on all aspects of orthopaedic surgery. It is the official journal of the Asia Pacific Orthopaedic Association. The journal welcomes and will publish materials of a diverse nature, from basic science research to clinical trials and surgical techniques. The journal encourages contributions from all parts of the world, but special emphasis is given to research of particular relevance to the Asia Pacific region.
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