Association of the Single Nucleotide Polymorphism 19216T/C in the TLR2 Gene (rs3804099) with Entamoeba histolytica/Entamoeba dispar/Entamoeba moshkovskii Infection Among Lebanese Children.

IF 1.9 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Israa Dib, Hiba Noureddine, Mohamad Fakih, Alexandre Livet, Vanessa Alphonse, Abbas Illayk, Abdallah Ahmad Medlej, Mahdi Tarhini, Noureddine Bousserrhine
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Abstract

Toll-like receptors (TLRs), particularly the TLR2, take part in the elicitation of immune responses against Entamoeba histolytica. This study aimed to investigate the relationship between a specific polymorphism called rs3804099 in the TLR2 gene and E. histolytica/E. dispar/E. moshkovskii infection among Lebanese children. A case-control study encompassed 180 participants including 68 children with amebiasis and 112 matched controls. Blood samples were collected, and genomic DNA was extracted using the classical proteinase K digestion and phenol-chloroform extraction method. The variant rs3804099 was examined using the Amplification Refractory Mutation System Polymerase Chain Reaction. The accuracy of the genotyping was supported by sequencing 5% of samples. The TLR2 rs3804099 polymorphism was identified in the studied population, and the observed genotypic distributions were consistent with Hardy-Weinberg equilibrium (P > 0.05). The frequency of the rare CC genotype was significantly higher in patients compared to the noninfected group (P < 0.01). In controls, the homozygous TT genotype was less frequent than the heterozygous CT genotype. The rare CC genotype was associated with a higher risk of amebiasis among children (odds ratios = 3.27, P = 0.002). These findings provide evidence supporting the association between the rs3804099 SNP in the TLR2 gene and E. histolytica/E. dispar/E. moshkovskii infection among Lebanese children.

TLR2基因中的单核苷酸多态性19216T/C(rs3804099)与黎巴嫩儿童中的组织溶解恩塔米巴/悬浮恩塔米巴/莫什科夫斯基恩塔米巴感染的关系。
Toll 样受体(TLRs),尤其是 TLR2,参与激发针对组织溶解恩塔莫阿米巴的免疫反应。本研究旨在调查 TLR2 基因中一种名为 rs3804099 的特定多态性与黎巴嫩儿童中的组织溶解性肠杆菌/E. dispar/E. moshkovskii 感染之间的关系。一项病例对照研究涵盖了 180 名参与者,包括 68 名阿米巴病儿童和 112 名匹配对照者。研究人员采集了血液样本,并使用经典的蛋白酶 K 消化法和苯酚-氯仿提取法提取了基因组 DNA。使用扩增难辨突变系统聚合酶链反应法检测了变异体 rs3804099。对 5%的样本进行测序证明了基因分型的准确性。在研究人群中发现了 TLR2 rs3804099 多态性,观察到的基因型分布与 Hardy-Weinberg 平衡一致(P > 0.05)。与非感染组相比,患者中罕见的 CC 基因型的频率明显更高(P < 0.01)。在对照组中,同型TT基因型的频率低于杂合CT基因型。罕见的 CC 基因型与儿童患阿米巴病的风险较高有关(几率比 = 3.27,P = 0.002)。这些发现为 TLR2 基因中的 rs3804099 SNP 与黎巴嫩儿童中的组织溶血性大肠杆菌/E. dispar/E. moshkovskii 感染之间的关联提供了证据。
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来源期刊
CiteScore
3.80
自引率
0.00%
发文量
78
审稿时长
2.2 months
期刊介绍: Journal of Interferon & Cytokine Research (JICR) provides the latest groundbreaking research on all aspects of IFNs and cytokines. The Journal delivers current findings on emerging topics in this niche community, including the role of IFNs in the therapy of diseases such as multiple sclerosis, the understanding of the third class of IFNs, and the identification and function of IFN-inducible genes.
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