Heat shock responsive genes in Brassicaceae: genome-wide identification, phylogeny, and evolutionary associations within and between genera.

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2024-12-01 Epub Date: 2024-10-16 DOI:10.1139/gen-2024-0061
Aldrin Y Cantila, Sheng Chen, Kadambot H M Siddique, Wallace A Cowling
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引用次数: 0

Abstract

Heat stress affects the growth and development of Brassicaceae crops. Plant breeders aim to mitigate the effects of heat stress by selecting for heat stress tolerance, but the genes responsible for heat stress in Brassicaceae remain largely unknown. During heat stress, heat shock proteins (HSPs) function as molecular chaperones to aid in protein folding, and heat shock transcription factors (HSFs) serve as transcriptional regulators of HSP expression. We identified 5002 heat shock related genes, including HSPs and HSFs, across 32 genomes in Brassicaceae. Among these, 3347 genes were duplicated, with segmented duplication primarily contributing to their expansion. We identified 466 physical gene clusters, including 240 homogenous clusters and 226 heterogeneous clusters, shedding light on the organization of heat shock related genes. Notably, 37 genes were co-located with published thermotolerance quantitative trait loci, which supports their functional role in conferring heat stress tolerance. This study provides a comprehensive resource for the identification of functional Brassicaceae heat shock related genes, elucidates their clustering and duplication patterns and establishes the genomic foundation for future heat tolerance research. We hypothesise that genetic variants in HSP and HSF genes in certain species have potential for improving heat stress tolerance in Brassicaceae crops.

十字花科植物的热休克反应基因:全基因组鉴定、系统发育以及属内和属间的进化关联。
热胁迫影响十字花科作物的生长和发育。植物育种者旨在通过选择热胁迫耐受性来减轻热胁迫的影响,但造成十字花科植物热胁迫的基因在很大程度上仍然未知。在热胁迫期间,热休克蛋白(HSPs)作为分子伴侣帮助蛋白质折叠,而热休克转录因子(HSFs)则是 HSP 表达的转录调节因子。我们在十字花科植物的 32 个基因组中发现了 5002 个热休克相关基因,包括 HSPs 和 HSFs。在这些基因中,有 3347 个基因是重复的,节段性重复是其扩展的主要原因。我们发现了 466 个物理基因簇,包括 240 个同质基因簇和 226 个异质基因簇,揭示了热休克相关基因的组织结构。值得注意的是,37 个基因与已发表的耐热性数量性状位点共定位,这支持了它们在赋予热胁迫耐受性方面的功能作用。这项研究为鉴定十字花科热休克相关功能基因提供了一个全面的资源,阐明了它们的聚类和重复模式,并为未来的耐热研究奠定了基因组基础。我们假设,某些物种中 HSP 和 HSF 基因的遗传变异有可能提高十字花科作物的热胁迫耐受性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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