Early intervention using long-term rhythmic pulsed magnetic stimulation alleviates cognitive decline in a 5xFAD mouse model of Alzheimer's disease

IF 4.6 2区医学 Q1 NEUROSCIENCES

Experimental Neurology Pub Date : 2024-10-16 DOI:10.1016/j.expneurol.2024.115002

Xue Wang , Qingmeng Wang , Xuting Wang , Haoyu Zhao , Chuncheng Zhao , Yangkun Jiao , Hongkai Shi , Changyou Chen , Haitao Chen , Pingping Wang , Tao Song

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引用次数: 0

Abstract

Background

Alzheimer's disease (AD) is the most prevalent form of dementia, but no effective therapeutic strategy is available to date. Rhythmic magnetic stimulation is an attractive means of neuron modulation that could be beneficial for restoring learning and memory abilities.

Objective

To assess the effect of a compound pulsed rhythmic magnetic field (cPMF) on cognition during AD progression and to explore the appropriate cPMF intervention period.

Methods

Female 5xFAD mice aged 10 weeks and 18 weeks were exposed to cPMF with a carrier frequency of 40 Hz, repeated at 5 Hz for 1 h/d for 8 consecutive weeks. The Morris water maze (MWM) test was used for cognitive behavioral assessment. Furthermore, changes in molecular pathology within the brain were detected using immunofluorescence staining and real-time PCR.

Results

10-week-old AD mice treated with cPMF explored the target quadrant more frequently than sham-exposed AD mice in MWM test, exhibiting improved learning and memory abilities. Additionally, cPMF exposure alleviated Aβ plaque deposition and astrogliosis in the AD brain. Moreover, neurotrophic factor fibroblast growth factor 1 (FGF1) in the AD brain was upregulated by cPMF treatment. However, in 18-week-old AD mice treated with cPMF, cognitive performance and Fgf1 gene expression were not significantly improved, although Aβ plaque deposition and astrogliosis were alleviated.

Conclusion

Early intervention via long-term rhythmic cPMF stimulation may alleviate the histopathological features and enhance neuroprotective gene Fgf1 expression, thereby improving the cognitive performance of 5xFAD mice, which should provide promising insight for the clinical treatment of patients with AD.

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使用长期节律性脉冲磁刺激进行早期干预可减轻 5xFAD 阿尔茨海默病小鼠模型的认知能力下降。

背景：阿尔茨海默病（AD）是最常见的痴呆症，但迄今为止尚无有效的治疗策略。有节奏的磁刺激是一种有吸引力的神经元调控手段，可能有益于恢复学习和记忆能力：目的：评估复合脉冲节奏磁场（cPMF）对AD进展过程中认知能力的影响，并探索cPMF的适当干预期：将年龄分别为 10 周和 18 周的雌性 5xFAD 小鼠暴露于载波频率为 40 Hz 的 cPMF 中，以 5 Hz 的频率重复 1 h/d，连续暴露 8 周。莫里斯水迷宫（MWM）测试用于认知行为评估。此外，还使用免疫荧光染色和实时 PCR 检测脑内分子病理学的变化：结果：与假暴露的AD小鼠相比，接受cPMF治疗的10周大AD小鼠在MWM测试中探索目标象限的频率更高，学习和记忆能力也有所提高。此外，暴露于cPMF可减轻AD脑中Aβ斑块沉积和星形胶质细胞增生。此外，AD大脑中的神经营养因子成纤维细胞生长因子1（FGF1）在cPMF处理后上调。然而，在接受cPMF治疗的18周龄AD小鼠中，虽然Aβ斑块沉积和星形胶质细胞病变有所缓解，但认知能力和Fgf1基因表达却没有明显改善：结论：通过长期有节律的cPMF刺激进行早期干预，可减轻组织病理学特征，提高神经保护基因Fgf1的表达，从而改善5xFAD小鼠的认知能力，这将为AD患者的临床治疗提供可喜的启示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental Neurology 医学-神经科学

CiteScore

10.10

自引率

3.80%

发文量

258

审稿时长

42 days

期刊介绍： Experimental Neurology, a Journal of Neuroscience Research, publishes original research in neuroscience with a particular emphasis on novel findings in neural development, regeneration, plasticity and transplantation. The journal has focused on research concerning basic mechanisms underlying neurological disorders.