Shining a light on the impact of antifungals on Aspergillus fumigatus subcellular dynamics through fluorescence imaging.

IF 4.1 2区 医学 Q2 MICROBIOLOGY
Antimicrobial Agents and Chemotherapy Pub Date : 2024-11-06 Epub Date: 2024-10-15 DOI:10.1128/aac.00803-24
I S R Storer, L E Sastré-Velásquez, T Easter, B Mertens, A Dallemulle, M Bottery, R Tank, M Offterdinger, M J Bromley, N van Rhijn, F Gsaller
{"title":"Shining a light on the impact of antifungals on <i>Aspergillus fumigatus</i> subcellular dynamics through fluorescence imaging.","authors":"I S R Storer, L E Sastré-Velásquez, T Easter, B Mertens, A Dallemulle, M Bottery, R Tank, M Offterdinger, M J Bromley, N van Rhijn, F Gsaller","doi":"10.1128/aac.00803-24","DOIUrl":null,"url":null,"abstract":"<p><p>Fluorescent proteins (FPs) are indispensable tools used for molecular imaging, single-cell dynamics, imaging in infection models, and more. However, next-generation FPs have yet to be characterized in <i>Aspergillus</i>. Here, we characterize 18 FPs in the pathogenic filamentous fungus <i>Aspergillus fumigatus</i> spanning the visible light spectrum. We report on <i>in vivo</i> FP brightness in hyphal and spore morphotypes and show how a fluoropyrimidine-based selection system can be used to iteratively introduce four distinct FPs enabling the simultaneous visualization of the cell membrane, mitochondria, peroxisomes, and vacuoles. Using this strain, we describe and compare the dynamic responses of organelles to stresses induced by voriconazole, amphotericin B, and the novel antifungal drugs olorofim and manogepix. The expansion to the fluorescent genetic toolbox will overcome boundaries in research applications that involve fluorescence imaging in filamentous fungi.</p>","PeriodicalId":8152,"journal":{"name":"Antimicrobial Agents and Chemotherapy","volume":" ","pages":"e0080324"},"PeriodicalIF":4.1000,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11539212/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antimicrobial Agents and Chemotherapy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1128/aac.00803-24","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/15 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Fluorescent proteins (FPs) are indispensable tools used for molecular imaging, single-cell dynamics, imaging in infection models, and more. However, next-generation FPs have yet to be characterized in Aspergillus. Here, we characterize 18 FPs in the pathogenic filamentous fungus Aspergillus fumigatus spanning the visible light spectrum. We report on in vivo FP brightness in hyphal and spore morphotypes and show how a fluoropyrimidine-based selection system can be used to iteratively introduce four distinct FPs enabling the simultaneous visualization of the cell membrane, mitochondria, peroxisomes, and vacuoles. Using this strain, we describe and compare the dynamic responses of organelles to stresses induced by voriconazole, amphotericin B, and the novel antifungal drugs olorofim and manogepix. The expansion to the fluorescent genetic toolbox will overcome boundaries in research applications that involve fluorescence imaging in filamentous fungi.

通过荧光成像揭示抗真菌药对烟曲霉亚细胞动力学的影响
荧光蛋白(FPs)是分子成像、单细胞动力学、感染模型成像等领域不可或缺的工具。然而,下一代荧光蛋白尚未在曲霉菌中得到表征。在这里,我们描述了致病性丝状真菌曲霉(Aspergillus fumigatus)中跨越可见光光谱的 18 种 FPs。我们报告了在菌丝和孢子形态中 FP 的活体亮度,并展示了如何利用基于氟嘧啶的选择系统迭代引入四种不同的 FP,从而实现细胞膜、线粒体、过氧化物酶体和液泡的同时可视化。利用这种菌株,我们描述并比较了细胞器对伏立康唑、两性霉素 B 以及新型抗真菌药物奥罗菲姆和马诺吉匹克诱导的压力的动态反应。荧光基因工具箱的扩展将突破丝状真菌荧光成像研究应用的界限。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
10.00
自引率
8.20%
发文量
762
审稿时长
3 months
期刊介绍: Antimicrobial Agents and Chemotherapy (AAC) features interdisciplinary studies that build our understanding of the underlying mechanisms and therapeutic applications of antimicrobial and antiparasitic agents and chemotherapy.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信