Rise in plasma bile acids following hypoabsorptive bariatric surgeries predicts beneficial metabolic and homeostatic outcomes in male rats.

IF 3.9 3区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY
Paulette Mukorako, Audrey-Anne Lavoie, Jocelyn Trottier, Natacha Lemoine, Laurent Biertho, Stéfane Lebel, Julie Plamondon, André Tchernof, David H St-Pierre, André Marette, Olivier Barbier, Denis Richard
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引用次数: 0

Abstract

This study was designed to investigate the effects of three hypoabsorptive bariatric surgeries, namely Roux-en-Y gastric bypass (RYGB), biliopancreatic diversion with duodenal switch (BPD-DS), and single anastomosis duodeno-ileal bypass with sleeve gastrectomy (SADI-S), on bile acids (BAs) and to assess whether the changes in BA plasma levels were associated with the metabolic and homeostatic effects of the surgeries. Male Wistar rats, either fed a high- (HF) or a low-fat (LF) diet, were divided into seven groups: RYGB HF, BPD-DS HF, SADI-S HF, sleeve-gastrectomy (SG) HF, sham-operated (Sham) HF, Sham LF, and Sham HF-pair-weighed to BPD-DS (Sham HF-PW). The rats were treated for 56 days. The results demonstrate the ability of RYGB, BPD-DS, and SADI-S to raise plasma levels of BAs, whose elevations were likely due to changes in gene expression of transporters, enzymes, and receptors in the liver and small intestine. This increase, most notably of the secondary BAs (deoxycholic acid, ursodeoxycholic acid, and lithocholic acid), was negatively associated with body weight gain, fat gain, and fasting insulin levels, and positively with plasma peptide tyrosine-tyrosine (PYY). Plasma BAs also correlated positively with the fecal levels of Clostridium, Sutterella, and Enterobacteriaceae and negatively with Clostridiales_f_g_2, Christensenellaceae, Ruminococcaceae_g_2, Oscillibacter, and Oscillospira. In addition, they are associated positively with the short-chain fatty acid (SCFA) levels of propionate, butyrate, isobutyrate, valerate, and isovalerate. Altogether, the present study emphasizes the ability of RYGB, BPD-DS, and SADI-S to induce circulating BA elevations that predict the beneficial consequences of those hypoabsorptive bariatric surgeries on energy and glucose homeostasis and circulating levels of PYY. The present results also reveal close associations between plasma BAs and SCFAs, whose variations following hypoabsorptive surgeries are linked to significant fat losses and metabolic health improvements.NEW & NOTEWORTHY The study emphasizes the ability of RYGB, BPD-DS, and SADI-S to induce elevated circulating bile acids levels and changes in the gene expression of transporters, enzymes and receptors in the liver and small intestine, predicting positive effects on energy and glucose homeostasis as well as PYY levels. The present results also reveal close associations between plasma BAs and SCFAs, whose variations following hypoabsorptive surgeries are also linked to significant fat losses and metabolic health improvements. These findings provide valuable insights into the mechanisms underlying the positive effects of these surgical interventions.

低吸收减肥手术后血浆胆汁酸的升高预示着雄性大鼠的代谢和体内平衡将得到改善。
本研究评估了三种低吸收性减肥手术(Roux-en-Y 胃旁路术(RYGB)、胆胰转流加十二指肠转换术(BPD-DS)和单吻合十二指肠-回肠旁路加袖状胃切除术(SADI-S))对胆汁酸(BAs)的影响,包括与手术的代谢和平衡效应相关的胆汁酸血浆水平的变化。雄性 Wistar 大鼠饲喂高脂(HF)或低脂(LF)饮食,分为七组:RYGB HF组、BPD-DS HF组、SADI-S HF组、袖状胃切除术(SG)HF组、假手术(SHAM)HF组、SHAM LF组和SHAM HF-BPD-DS组(SHAM HF-PW)。大鼠共接受了 56 天的治疗。结果表明,RYGB、BPD-DS 和 SADI-S 能够提高血浆胆碱酯酶水平,其升高,尤其是次级胆碱酯酶(脱氧胆酸、熊去氧胆酸和石胆酸)的升高与体重增加、脂肪增加和空腹胰岛素水平呈负相关,而与血浆肽酪氨酸-酪氨酸(PYY)呈正相关。血浆 BAs 还与粪便中的梭状芽孢杆菌、沙氏菌和肠杆菌呈正相关,与梭状芽孢杆菌_f_g_2、克里斯滕森菌科、反刍球菌_g_2、弧菌和弧菌呈负相关。此外,它们还与丙酸、丁酸、异丁酸、戊酸和异戊酸等短链脂肪酸(SCFA)水平呈正相关。总之,本研究强调了 RYGB、BPD-DS 和 SADI-S 诱导循环 BA 升高的能力,这预示着这些低吸收减肥手术会对能量和葡萄糖稳态以及循环中的PYY 水平产生有益的影响。本研究结果还揭示了血浆 BA 和 SCFA 之间的密切联系,它们在低吸收手术后的变化也与脂肪的显著减少和代谢健康的改善有关。
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来源期刊
CiteScore
9.40
自引率
2.20%
发文量
104
审稿时长
1 months
期刊介绍: The American Journal of Physiology-Gastrointestinal and Liver Physiology publishes original articles pertaining to all aspects of research involving normal or abnormal function of the gastrointestinal tract, hepatobiliary system, and pancreas. Authors are encouraged to submit manuscripts dealing with growth and development, digestion, secretion, absorption, metabolism, and motility relative to these organs, as well as research reports dealing with immune and inflammatory processes and with neural, endocrine, and circulatory control mechanisms that affect these organs.
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