Detection of extended-spectrum beta-lactamase genes among Escherichia coli isolates of buffalo mastitis milk

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics Pub Date : 2024-10-05 DOI:10.1016/j.egg.2024.100297

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Abstract

In recent years, bacteria carrying extended-spectrum beta-lactamase (ESBL) genes have become increasingly prevalent. These genes provide resistance to antibiotics, making treatment more challenging. Escherichia coli is a major cause of mastitis in buffaloes, and there have been reports of ESBL genes in E. coli isolated from bovine mastitis milk. This study was aimed to detect ESBL genes in E. coli found in mastitis buffalo milk. A total of 100 samples of mastitis milk were collected from different buffalo farms in Karachi for gene detection and antibiotic sensitivity evaluation. Among the samples, 44 % were positive for E. coli, confirmed by specific characteristics and biochemical properties. To check the antimicrobial sensitivity of isolates MIC test was carried out using micro broth dilution method. Antibiotic sensitivity tests revealed that out of the 44 isolates, 68 % were resistant to ampicillin, 81 % to tetracycline, 52 % to levofloxacin, 27 % to cefoxitin, and 25 % to ceftriaxone at various concentrations. Additionally, 7 % of the isolates showed resistance to all classes of antibiotics tested. PCR results indicated that 25 % of the total isolates carried the blaCTX-M-1 ESBL gene, while no other types of blaCTX-M, blaSHV, or blaTEM genes were detected. It was discovered that multidrug resistance and the presence of the blaCTX-M-1 ESBL gene in E. coli isolates from mastitis milk pose a significant threat to veterinarians and human clinicians, as they are highly resistant to beta-lactams and other commonly used antibiotics for mastitis treatment.

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检测水牛乳腺炎牛奶中分离出的大肠埃希菌的广谱β-内酰胺酶基因

近年来，携带广谱β-内酰胺酶（ESBL）基因的细菌越来越多。这些基因会对抗生素产生抗药性，使治疗更具挑战性。大肠埃希菌是导致水牛乳腺炎的主要原因，有报道称从牛乳腺炎牛奶中分离出的大肠埃希菌含有 ESBL 基因。本研究旨在检测乳腺炎水牛奶中大肠杆菌的 ESBL 基因。研究人员从卡拉奇不同的水牛养殖场共采集了 100 份乳腺炎牛奶样本，用于基因检测和抗生素敏感性评估。经特异性特征和生化特性确认，样本中有 44% 的大肠杆菌呈阳性。为了检测分离物的抗菌敏感性，采用了微量肉汤稀释法进行 MIC 测试。抗生素敏感性测试表明，在 44 个分离菌株中，有 68% 对氨苄西林耐药，81% 对四环素耐药，52% 对左氧氟沙星耐药，27% 对头孢西丁耐药，25% 对不同浓度的头孢曲松耐药。此外，有 7% 的分离菌株对所有测试抗生素都表现出耐药性。聚合酶链式反应（PCR）结果表明，25%的分离株携带 blaCTX-M-1 ESBL 基因，而没有检测到其他类型的 blaCTX-M、blaSHV 或 blaTEM 基因。研究发现，乳腺炎牛奶中分离出的大肠杆菌对β-内酰胺类和其他治疗乳腺炎的常用抗生素具有很强的抗药性，因此，大肠杆菌的多药耐药性和 blaCTX-M-1 ESBL 基因的存在对兽医和人类临床医生构成了重大威胁。

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来源期刊

Ecological Genetics and Genomics Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： Ecological Genetics and Genomics publishes ecological studies of broad interest that provide significant insight into ecological interactions or/ and species diversification. New data in these areas are published as research papers, or methods and resource reports that provide novel information on technologies or tools that will be of interest to a broad readership. Complete data sets are shared where appropriate. The journal also provides Reviews, and Perspectives articles, which present commentary on the latest advances published both here and elsewhere, placing such progress in its broader biological context. Topics include: -metagenomics -population genetics/genomics -evolutionary ecology -conservation and molecular adaptation -speciation genetics -environmental and marine genomics -ecological simulation -genomic divergence of organisms