Immunohistochemical distribution of cannabinoid receptor type 1 (CB1) and type 2 (CB2) in the rat carotid body

IF 2.3 4区生物学 Q4 CELL BIOLOGY

Acta histochemica Pub Date : 2024-10-13 DOI:10.1016/j.acthis.2024.152205

Hiroki Saito, Takuya Yokoyama , Nobuaki Nakamuta, Yoshio Yamamoto

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引用次数: 0

Abstract

The carotid body is a hypoxia-sensitive chemoreceptor that induces sensory long-term facilitation after exposure to chronic intermittent hypoxia. However, the mechanisms underlying synaptic plasticity in the carotid body remain unknown. In the present study, we examined the immunohistochemical distribution of cannabinoid receptor type 1 (CB1) and type 2 (CB2), which are candidate molecules involved in the modulation of synaptic transmission. Dot-like CB1 immunoreactivity was distributed in the perinuclear cytoplasm of chemoreceptor cells immunoreactive for the catecholamine-synthesizing enzymes, tyrosine hydroxylase and dopamine beta-hydroxylase. Furthermore, CB1 immunoreactivity was observed in sensory nerve endings immunoreactive for P2X₃ purinoceptors that colocalized with vesicular glutamate transporter 2. On the other hand, immunoreactivity for CB2 was mainly distributed in chemoreceptor cells, and was weakly observed in sensory nerve endings immunoreactive for P2X₂ purinoceptors. The present results suggest that CB1 and CB2 regulate the release of catecholamines and glutamate from chemoreceptor cells and sensory nerve endings, respectively. Therefore, CB1 and CB2 may be involved in synaptic plasticity in the carotid body.

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大鼠颈动脉体中大麻素受体 1 型（CB1）和 2 型（CB2）的免疫组化分布

颈动脉体是一种对缺氧敏感的化学感受器，在暴露于慢性间歇性缺氧后会诱发感觉的长期促进。然而，颈动脉体突触可塑性的机制仍然未知。在本研究中，我们检测了大麻素受体 1 型（CB1）和 2 型（CB2）的免疫组化分布，它们是参与调节突触传递的候选分子。点状 CB1 免疫活性分布在对儿茶酚胺合成酶、酪氨酸羟化酶和多巴胺 beta-羟化酶有免疫活性的化学感受器细胞的核周细胞质中。此外，在对 P2X3 嘌呤受体有免疫反应的感觉神经末梢也观察到了 CB1 免疫反应，这些受体与囊泡谷氨酸转运体 2 共同定位。另一方面，CB2 的免疫反应主要分布在化学感受器细胞中，在对 P2X2 嘌呤受体免疫反应的感觉神经末梢中观察到微弱的 CB2 免疫反应。本研究结果表明，CB1 和 CB2 分别调节化学感受器细胞和感觉神经末梢儿茶酚胺和谷氨酸的释放。因此，CB1 和 CB2 可能参与了颈动脉体的突触可塑性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta histochemica 生物-细胞生物学

CiteScore

4.60

自引率

4.00%

发文量

107

审稿时长

23 days

期刊介绍： Acta histochemica, a journal of structural biochemistry of cells and tissues, publishes original research articles, short communications, reviews, letters to the editor, meeting reports and abstracts of meetings. The aim of the journal is to provide a forum for the cytochemical and histochemical research community in the life sciences, including cell biology, biotechnology, neurobiology, immunobiology, pathology, pharmacology, botany, zoology and environmental and toxicological research. The journal focuses on new developments in cytochemistry and histochemistry and their applications. Manuscripts reporting on studies of living cells and tissues are particularly welcome. Understanding the complexity of cells and tissues, i.e. their biocomplexity and biodiversity, is a major goal of the journal and reports on this topic are especially encouraged. Original research articles, short communications and reviews that report on new developments in cytochemistry and histochemistry are welcomed, especially when molecular biology is combined with the use of advanced microscopical techniques including image analysis and cytometry. Letters to the editor should comment or interpret previously published articles in the journal to trigger scientific discussions. Meeting reports are considered to be very important publications in the journal because they are excellent opportunities to present state-of-the-art overviews of fields in research where the developments are fast and hard to follow. Authors of meeting reports should consult the editors before writing a report. The editorial policy of the editors and the editorial board is rapid publication. Once a manuscript is received by one of the editors, an editorial decision about acceptance, revision or rejection will be taken within a month. It is the aim of the publishers to have a manuscript published within three months after the manuscript has been accepted