Genetic diversity and structure of Tunisian and Indian date palm (Phoenix dactylifera and sylvestris) cultivars and genotypes revealed by AFLP markers

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics Pub Date : 2024-10-09 DOI:10.1016/j.egg.2024.100299

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引用次数: 0

Abstract

The date palm breeding programs need to discover valid genetic fingerprints to characterize cultivars and assess their genetic diversity. This study assessed the genetic diversity among thirty-nine date palm cultivars from Tunisia (Phoenix dactylifera) and India (Phoenix sylvestris) by using six AFLP (Amplified Fragment Length Polymorphism) markers. 360 loci were amplified, with 127 loci polymorphic (34.35 %). The Jaccard's similarity coefficient ranged from 0.161 to 0.931, with the mean genetic distances of 0.568. AFLP's average marker index value was 7.28, with a resolving power of 10.91. The analysis of population structure showed two main clusters with a clear separation between Tunisian and Indian cultivars.

Furthermore, the heatmap analysis allowed the identification of 10 bands specific to the Indian accessions, which were not detected in Tunisian genotypes. These loci could be linked to genes involved in adapting the species in Indian lands, which allowed the study of the genetic diversity of date palm resources of different origins, confirming the existence of at least two origins of domestication. Additionally, identifying AFLP loci specific to P. dactylifera and P. sylvestris will significantly contribute to breeding programs by exploiting species-specific polymorphisms.

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用 AFLP 标记揭示突尼斯和印度枣椰树（Phoenix dactylifera 和 sylvestris）栽培品种和基因型的遗传多样性和结构

枣椰树育种计划需要发现有效的遗传指纹，以确定栽培品种的特征并评估其遗传多样性。本研究利用六个 AFLP（扩增片段长度多态性）标记，评估了来自突尼斯（Phoenix dactylifera）和印度（Phoenix sylvestris）的 39 个椰枣栽培品种的遗传多样性。共扩增了 360 个位点，其中 127 个位点具有多态性（34.35%）。Jaccard 相似度系数在 0.161 至 0.931 之间，平均遗传距离为 0.568。AFLP 的平均标记指数值为 7.28，分辨率为 10.91。种群结构分析表明，突尼斯栽培品种和印度栽培品种之间有两个明显区分的主要聚类。此外，热图分析还发现了 10 个印度品种特有的条带，这些条带在突尼斯基因型中没有检测到。这些基因位点可能与该物种在印度土地上的适应基因有关，这有助于研究不同来源的枣椰树资源的遗传多样性，证实至少存在两个驯化起源。此外，确定 P. dactylifera 和 P. sylvestris 的特异性 AFLP 位点将通过利用物种特异性多态性为育种计划做出重大贡献。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ecological Genetics and Genomics Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： Ecological Genetics and Genomics publishes ecological studies of broad interest that provide significant insight into ecological interactions or/ and species diversification. New data in these areas are published as research papers, or methods and resource reports that provide novel information on technologies or tools that will be of interest to a broad readership. Complete data sets are shared where appropriate. The journal also provides Reviews, and Perspectives articles, which present commentary on the latest advances published both here and elsewhere, placing such progress in its broader biological context. Topics include: -metagenomics -population genetics/genomics -evolutionary ecology -conservation and molecular adaptation -speciation genetics -environmental and marine genomics -ecological simulation -genomic divergence of organisms