First use of Chironomid Pupal Exuvial Technique in freshwater biomonitoring in Cameroon: Ecological aspect and morphological description of Chironomidae (Insecta: Diptera)

Abstract

The biodiversity of urban streams is gradually degrading as a result of anthropogenic pollution. In this study, chironomid pupal exuviae were used as surrogates to assess urban streams and a lake in Douala Cameroon. We compared these assessments with samples collected from a suburban forest stream. Based on their highly adaptive capability, we predicted that chironomid communities would present high diversity and abundance in urban habitats. Chironomid pupal exuviae were collected using the chironomid pupal exuvial technique in 14 urban and 2 forest reference stations during dry and rainy seasons. Measurements of the environmental variables were done simultaneously. Physicochemical analysis revealed that all urban sites have had very poor ecological health status whereas forest site had good water quality as shown by the hierarchical cluster analysis. This ecological survey identified 8 species/morphotypes of chironomids all belonging to the subfamily Chironominae, tribe Chironomini. These are Chironomus holomelas(?), Chironomus sp.1, Chironomus sp.2, Chironomus sp.3, Chironomus (Lobochironomus) sp., Stenochironomus sp., Dicrotendipes sp. and Dicrotendipes pulsus. No midge pupal exuviae were recorded at the forest stations (S₁ & S₂) and the stations S₆ and S₇ located downstream industrial mill effluents. The species richness of the study sites was significantly very low as compare to other Afrotropical studies. Canonical redundancy analysis revealed that high values of water temperature, ammonium, nitrates and phosphates, and low oxygen concentration were significant predictors of the distribution and high abundance of Chironomidae. This survey highlighted the impacts of anthropogenic activities on chironomid diversity and distribution, with great decrease of their diversity due to industrial pollution.