Assessment of mammary stress in early lactating crossbred (Alpine × Beetal) does during heat stress in conjunction with milk somatic cells and non-invasive indicators

Abstract

Milk somatic cell counts (SCC) are crucial for monitoring dairy animals' mammary health and milk quality. This study was initiated to assess the mammary health of early lactating crossbred (Alpine × Beetal) does during periods of heat stress. The udder skin surface temperature (USST), total and differential milk SCC, milk composition, milk cortisol levels and phagocytic activity (PA) of milk neutrophils and macrophages were estimated in these does. Results indicated a significant (p<0.01) increase in the milk neutrophils to macrophages (N: M) ratio and milk cortisol levels with milk somatic cell score (SCS). The PA of milk neutrophils decreased significantly (p<0.01), while that of macrophages increased significantly (p<0.01) with higher SCC. On Pearson correlation, milk SCS revealed strong positive correlation (p<0.01) with N: M ratio (r=0.90), USST (r=0.98), milk cortisol (r=0.97), milk pH (r=0.83) and conductivity (r=0.92). Conversely, milk lactose exhibited a moderate negative correlation of (r= −0.48) with SCS. These findings suggest that USST infrared thermography and milk cortisol levels are promising non-invasive methods for rapidly assessing mammary stress in early lactating does during heat stress.