In-situ polyherbal gel as biomedicine in the management of Alzheimer's disease: Understanding ameliorative potential in Trimethyltin induced neurodegeneration

IF 1.3 4区医学 Q4 PHARMACOLOGY & PHARMACY

Journal of pharmacological and toxicological methods Pub Date : 2024-10-10 DOI:10.1016/j.vascn.2024.107567

Sneha Latha Diddi , Sathiyanarayanan Lohidasan , Arulmozhi S , Vividha Dhapte-Pawar , Kakasaheb R. Mahadik

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引用次数: 0

Abstract

Alzheimer's disease (AD), classified as neurodegenerative disorder that progresses over a period of time, is characterized by intracellular neurofibrillary tangles and extracellular amyloid plaques. This present research work was designed to develop a polyherbal gel for the treatment of AD. This research study is aimed to confirm the impact and validation of polyherbal gel on tauopathy and neurodegeneration that had been induced by intraperitoneal trimethyltin (TMT) injection to rats.

Polyherbal loaded gel was prepared by cold method, and characterized for gel strength, viscosity, permeation and pH. Subsequently, 5 marker based standardized plant materials of Kalyanka ghrita were incorporated in gellan gum and xanthan gum. Finally, an in-vivo investigation employing rats with TMT-induced neurological disease were used to assess the efficacy of the optimized gel.

On day 7, the Wistar rats received intraperitoneal injections of TMT. From day 14 to day 35, the corresponding groups received intranasal administration of polyherbal gel. In addition to the molecular parameters such as brain acetyl cholinesterase activity, BDNF (Rat brain derived neurotropic factor), protein phosphatase 2 A, antioxidant parameters, and oxidative stress markers, the behavioral parameters were also determined. Studies were conducted on the brain's monoamine levels and histology.

Results

Higher permeation over the nasal mucosa was demonstrated by the optimized In-situ polyherbal gel. Significant improvement in cognition was observed from the reduced escape latency, longer paths, and increased social or novel object recognition tests post polyherbal gel treatment. A documented HPLC technique helped in optimization and standardization of the polyherbal gel. The polyherbal treatment groups exhibited a considerable rise in the levels of monoamines, including norepinephrine, dopamine, and 5-hydroxy tryptamine.

Conclusion

According to the current study, treating Alzheimer's disease (AD) with a polyherbal gel formulation may be a viable option for successful therapy.

查看原文本刊更多论文

将原位多草药凝胶作为治疗阿尔茨海默氏症的生物医药：了解三甲基锡诱导的神经变性的改善潜力。

阿尔茨海默病（AD）被归类为神经退行性疾病，会在一段时间内发展，其特征是细胞内的神经纤维缠结和细胞外的淀粉样斑块。本研究工作旨在开发一种多草药凝胶，用于治疗 AD。这项研究旨在证实多草本凝胶对大鼠腹腔注射三甲基锡（TMT）诱导的牛头蛋白病和神经退行性变的影响和有效性。多草本凝胶采用冷冻法制备，并对凝胶强度、粘度、渗透性和 pH 值进行了表征。随后，在结冷胶和黄原胶中加入了 5 种基于 Kalyanka ghrita 标准化植物材料的标记物。最后，使用 TMT 诱导的神经系统疾病大鼠进行体内调查，以评估优化凝胶的功效。第 7 天，Wistar 大鼠腹腔注射 TMT。从第 14 天到第 35 天，相应组别接受多草本凝胶的鼻内给药。除了脑乙酰胆碱酯酶活性、BDNF（大鼠脑源性神经营养因子）、蛋白磷酸酶 2 A、抗氧化参数和氧化应激标记物等分子参数外，还测定了行为参数。还对大脑单胺水平和组织学进行了研究。结果：优化后的原位多草药凝胶在鼻粘膜上的渗透性更高。多草药凝胶治疗后，逃逸潜伏期缩短，路径延长，社交或新物体识别测试增加，从而观察到认知能力显著提高。有据可查的高效液相色谱技术有助于多草本凝胶的优化和标准化。多草药治疗组的单胺类物质（包括去甲肾上腺素、多巴胺和 5-羟色胺）水平显著上升。结论：根据目前的研究，用多草本凝胶配方治疗阿尔茨海默病（AD）可能是成功治疗的可行选择。

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来源期刊

Journal of pharmacological and toxicological methods PHARMACOLOGY & PHARMACY-TOXICOLOGY

CiteScore

3.60

自引率

10.50%

发文量

审稿时长

26 days

期刊介绍： Journal of Pharmacological and Toxicological Methods publishes original articles on current methods of investigation used in pharmacology and toxicology. Pharmacology and toxicology are defined in the broadest sense, referring to actions of drugs and chemicals on all living systems. With its international editorial board and noted contributors, Journal of Pharmacological and Toxicological Methods is the leading journal devoted exclusively to experimental procedures used by pharmacologists and toxicologists.