Cyclooxygenase and lipoxygenase metabolites during platelet aggregation: Quantitative measurement by negative ion chemical ionization — gas chromatography / mass spectrometry

E. Malle , J. Nimpf , H.J. Leis , H. Wurm , H. Gleispach , G.M. Kostner
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引用次数: 21

Abstract

This study was aimed at investigating systemetically the aggregation of human gel filtered platelets induced by various physiological stimuli such as thrombin (0.25 U/ml), collagen (2 μg/ml), a mixture of thrombin and collagen, and ADP (2–5 μM). For quantitative measurement of TXB2, PGF2a and 12-HETE, negative ion chemical ionization - gas chromatography/mass spectrometry using stable isotope dilution was applied. Stimulation by thrombin, collagen and the combined agonists resulted in an increase of TXB2 (up to 54 ng/1 × 108 platelets) and 12-HETE (up to 44 ng/1 × 108 platelets) during platelet aggregation. The simulation with ADP showed only little increase of these metabolites and this only during the second wave of aggregation. Very little amounts of PGF2α were produced during thrombin stimulated aggregation.

血小板聚集过程中的环加氧酶和脂加氧酶代谢物:负离子化学电离-气相色谱/质谱法定量测定
本研究旨在系统研究凝血酶(0.25 U/ml)、胶原蛋白(2 μg/ml)、凝血酶和胶原蛋白的混合物、ADP (2 - 5 μM)等生理刺激对人凝胶滤过血小板聚集的影响。采用稳定同位素稀释负离子化学电离-气相色谱/质谱法定量测定TXB2、PGF2a和12-HETE。凝血酶、胶原蛋白和联合激动剂的刺激导致血小板聚集过程中TXB2(高达54 ng/1 × 108血小板)和12-HETE(高达44 ng/1 × 108血小板)的增加。ADP模拟显示,这些代谢物仅在第二波聚集期间略有增加。在凝血酶刺激的聚集过程中产生少量的PGF2α。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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