Online monitoring of methane transfer rates unveils nitrogen fixation dynamics in Methylococcus capsulatus.

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Dominik Engel, Maximilian Hoffmann, Udo Kosfeld, Marcel Mann
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引用次数: 0

Abstract

This study explores methane utilization by the methanotrophic microorganism Methylococcus capsulatus (Bath) for biomass production, presenting a promising approach to mitigate methane emissions and foster the development sustainable biomaterials. Traditional screening methods for gas cultivations involve either serum flasks without online monitoring or costly, low-throughput fermenters. To address these limitations, the Respiration Activity MOnitoring System was augmented with methane sensors for real-time methane transfer rate (MTR) monitoring in shake flasks. Utilizing online monitoring of the MTR in shake flasks results in enhanced throughput and cost-effectiveness for cultivating M. capsulatus. Simultaneous monitoring of transfer rates for oxygen, methane, and carbon dioxide was conducted in up to eight shake flasks, ensuring the success of the cultivation process. Alterations in methane-to-oxygen transfer rate ratios and carbon fixation rates reveal the impact of transfer limitations on microbial growth. Detection of gas transfer limitations, exploration of process parameter influences, and investigations of medium components were enabled by the introduced method. Optimal nitrogen concentrations could be determined to ensure optimal growth. This streamlined approach accelerates the screening process, offering efficient investigations into metabolic effects, limitations, and parameter influences in gas fermentations without the need for elaborate offline sampling, significantly reducing costs and enhanced reproducibility.

甲烷转移率的在线监测揭示了荚膜甲球菌的固氮动态。
本研究探讨了甲烷营养微生物荚膜甲烷球菌(Bath)利用甲烷生产生物质的情况,为减少甲烷排放和促进可持续生物材料的发展提供了一种前景广阔的方法。传统的气体培养筛选方法涉及没有在线监测的血清烧瓶或昂贵的低通量发酵罐。为了解决这些局限性,呼吸活动监测系统增加了甲烷传感器,用于实时监测摇瓶中的甲烷转移率(MTR)。利用在线监测摇瓶中的甲烷转移率可提高培养蝙蝠蛾的产量和成本效益。在多达八个摇瓶中同时监测氧气、甲烷和二氧化碳的转移率,确保了培养过程的成功。甲烷与氧气的转移率比率和碳固定率的变化揭示了转移限制对微生物生长的影响。采用这种方法可以检测气体转移限制、探索过程参数的影响以及研究培养基成分。可以确定最佳氮浓度,以确保最佳生长。这种简化方法加快了筛选过程,可有效研究气体发酵中的代谢作用、限制和参数影响,而无需进行复杂的离线采样,从而大大降低了成本并提高了可重复性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biotechnology and Bioengineering
Biotechnology and Bioengineering 工程技术-生物工程与应用微生物
CiteScore
7.90
自引率
5.30%
发文量
280
审稿时长
2.1 months
期刊介绍: Biotechnology & Bioengineering publishes Perspectives, Articles, Reviews, Mini-Reviews, and Communications to the Editor that embrace all aspects of biotechnology. These include: -Enzyme systems and their applications, including enzyme reactors, purification, and applied aspects of protein engineering -Animal-cell biotechnology, including media development -Applied aspects of cellular physiology, metabolism, and energetics -Biocatalysis and applied enzymology, including enzyme reactors, protein engineering, and nanobiotechnology -Biothermodynamics -Biofuels, including biomass and renewable resource engineering -Biomaterials, including delivery systems and materials for tissue engineering -Bioprocess engineering, including kinetics and modeling of biological systems, transport phenomena in bioreactors, bioreactor design, monitoring, and control -Biosensors and instrumentation -Computational and systems biology, including bioinformatics and genomic/proteomic studies -Environmental biotechnology, including biofilms, algal systems, and bioremediation -Metabolic and cellular engineering -Plant-cell biotechnology -Spectroscopic and other analytical techniques for biotechnological applications -Synthetic biology -Tissue engineering, stem-cell bioengineering, regenerative medicine, gene therapy and delivery systems The editors will consider papers for publication based on novelty, their immediate or future impact on biotechnological processes, and their contribution to the advancement of biochemical engineering science. Submission of papers dealing with routine aspects of bioprocessing, description of established equipment, and routine applications of established methodologies (e.g., control strategies, modeling, experimental methods) is discouraged. Theoretical papers will be judged based on the novelty of the approach and their potential impact, or on their novel capability to predict and elucidate experimental observations.
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