Exploring MPC1 as a potential ferroptosis-linked biomarker in the cervical cancer tumor microenvironment: a comprehensive analysis.

IF 3.4 2区医学 Q2 ONCOLOGY

BMC Cancer Pub Date : 2024-10-10 DOI:10.1186/s12885-024-12622-x

Miao Li, Tianhan Xu, Rui Yang, Xiaoyun Wang, Jiawen Zhang, Sufang Wu

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引用次数: 0

Abstract

Background: The increasing problems of drug and radiotherapy resistance in cervical cancer underscores the need for novel methods for its management. Reports indicate that the expression of MPC1 may be associated with the tumor microenvironment and the occurrence of ferroptosis in cervical cancer. The objective of this study was to visually illustrate the prognostic significance and immunological characterization of MPC1 in cervical cancer.

Methods: The expression profile and prognostic significance of MPC1 were analyzed using various databases, including UALCAN, TIMER2, GEPIA2, and Kaplan-Meier Plotter. TISIDB, TIMER2, and immunohistochemical analysis were used to investigate the correlation between MPC1 expression and immune infiltration. GO enrichment analysis, KEGG analysis, Reactome analysis, ConsensusPathDB, and GeneMANIA were used to visualize the functional enrichment of MPC1 and signaling pathways related to MPC1. The correlation analysis was carried out to examine the relationship between MPC1 and Ferroptosis gene in TIMER 2.0, ncFO, GEPIA Database and Kaplan-Meier Plotter.

Results: We demonstrated that the expression levels of MPC1 in cervical cancer tissues were lower than those in normal cervical tissues. Kaplan-Meier survival curves showed shorter overall survival in cervical cancer patients with low levels of MPC1 expression. The expression of MPC1 was related to the infiltrating levels of tumor-infiltrating immune cells in cervical cancer. Moreover, MPC1 expression was associated with the iron-mediated cell death pathway, and several important ferroptosis genes were upregulated in cervical cancer cells. Furthermore, after knocking down MPC1 in HeLa cells, the expression of these genes decreased.

Conclusion: These findings indicate that MPC1 functions as a prognostic indicator and plays a role in the regulation of the ferroptosis pathway in cervical cancer.

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探索宫颈癌肿瘤微环境中潜在的铁突变相关生物标记物 MPC1：一项综合分析。

背景：宫颈癌耐药和耐放疗的问题日益严重，这凸显了对新型治疗方法的需求。有报告显示，MPC1 的表达可能与宫颈癌的肿瘤微环境和铁变态反应的发生有关。本研究旨在直观地说明 MPC1 在宫颈癌中的预后意义和免疫学特征：方法：利用 UALCAN、TIMER2、GEPIA2 和 Kaplan-Meier Plotter 等多个数据库分析了 MPC1 的表达谱和预后意义。TISIDB、TIMER2和免疫组化分析用于研究MPC1表达与免疫浸润之间的相关性。GO富集分析、KEGG分析、Reactome分析、ConsensusPathDB和GeneMANIA用于观察MPC1及与MPC1相关的信号通路的功能富集。在TIMER 2.0、ncFO、GEPIA数据库和Kaplan-Meier Plotter中进行了相关性分析，以研究MPC1与铁突变基因之间的关系：结果：我们发现宫颈癌组织中 MPC1 的表达水平低于正常宫颈组织。Kaplan-Meier生存曲线显示，MPC1表达水平低的宫颈癌患者总生存期较短。MPC1的表达与宫颈癌中肿瘤浸润免疫细胞的浸润水平有关。此外，MPC1的表达与铁介导的细胞死亡途径有关，宫颈癌细胞中几个重要的铁变态反应基因被上调。此外，在敲除 HeLa 细胞中的 MPC1 后，这些基因的表达量减少：这些研究结果表明，MPC1 可作为预后指标，并在宫颈癌的铁氧化途径调控中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Cancer 医学-肿瘤学

CiteScore

6.00

自引率

2.60%

发文量

1204

审稿时长

6.8 months

期刊介绍： BMC Cancer is an open access, peer-reviewed journal that considers articles on all aspects of cancer research, including the pathophysiology, prevention, diagnosis and treatment of cancers. The journal welcomes submissions concerning molecular and cellular biology, genetics, epidemiology, and clinical trials.