STUDY ON THE ROLE AND MECHANISM OF MICRORNA-650/WNT1 IN THE REPAIR OF ARTICULAR CARTILAGE INJURY.

IF 0.5 4区医学 Q4 ORTHOPEDICS

Acta Ortopedica Brasileira Pub Date : 2024-10-07 eCollection Date: 2024-01-01 DOI:10.1590/1413-785220243204e278218

Hui Liu, Yue Wang, Shuyuan Wang, Bo Yang, Di Sun, Shuangyang Han

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引用次数: 0

Abstract

Objectives: Osteoarthritis (OA) is a degenerative disease associated with chondrocyte injury. This study investigated the dysregulation of microRNA-650 (miR-650) in cartilage tissues of patients with OA. Its function and mechanism were also investigated in OA cell models.

Methods: miR-650 levels were examined in 15 OA cartilage tissues and ten healthy cartilage tissues. SW1353 cells were used for cell function experiments and IL-1β was applied to the cells to mimic OA conditions in vitro. Cell functions such as proliferation, apoptosis, and inflammation were detected. The downstream target gene of miR-650 was identified and confirmed by bioinformatic analysis and luciferase activity assay. Rescue experiments were performed to verify the mechanism.

Results: Suppressed expression of miR-650 was tested in patients with OA and cell models. Overexpression of miR-650 increased cell proliferation but suppressed apoptosis and inflammation of SW1353. As the target gene of miR-650, WNT1 overexpression counteracted the role of miR-650 in the function of SW1353.

Conclusion: miR-650 can protect against articular cartilage injury in OA by targeting WNT1. Level of Evidence I, Experimental Study.

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研究 microrna-650/wnt1 在关节软骨损伤修复中的作用和机制。

目的：骨关节炎（OA）是一种与软骨细胞损伤有关的退行性疾病。本研究调查了OA患者软骨组织中microRNA-650（miR-650）的失调情况。方法：研究人员检测了 15 个 OA 软骨组织和 10 个健康软骨组织中的 miR-650 水平。方法：研究人员检测了 15 个 OA 软骨组织和 10 个健康软骨组织中 miR-650 的水平。实验检测了细胞的增殖、凋亡和炎症等功能。通过生物信息学分析和荧光素酶活性测定，确定并确认了 miR-650 的下游靶基因。为验证其机制，还进行了拯救实验：结果：在 OA 患者和细胞模型中检测了 miR-650 的抑制表达。过表达 miR-650 增加了 SW1353 的细胞增殖，但抑制了细胞凋亡和炎症反应。作为 miR-650 的靶基因，WNT1 的过表达抵消了 miR-650 在 SW1353 功能中的作用。证据等级 I，实验研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Ortopedica Brasileira Orthopedics-

CiteScore

0.90

自引率

14.30%

发文量

审稿时长

25 weeks

期刊介绍： A Revista Acta Ortopédica Brasileira, órgão oficial do Departamento de Ortopedia e Traumatologia da Faculdade de Medicina da Universidade de São Paulo (DOT/FMUSP), é publicada bimestralmente em seis edições ao ano (jan/fev, mar/abr, maio/jun, jul/ago, set/out e nov/dez) com versão em inglês disponível nos principais indexadores nacionais e internacionais e instituições de ensino do Brasil. Sendo hoje reconhecidamente uma importante contribuição para os especialistas da área com sua seriedade e árduo trabalho para as indexações já conquistadas.