Location of polyadenylation sites within 3' untranslated regions is linked to biological function in yeast.

IF 3.3 3区生物学 Q2 GENETICS & HEREDITY

Genetics Pub Date : 2024-10-09 DOI:10.1093/genetics/iyae163

Joseph V Geisberg, Zarmik Moqtaderi, Kevin Struhl

引用次数: 0

Abstract

Expression of a typical yeast gene results in ∼50 3' mRNA isoforms that are distinguished by the locations of poly(A) sites within the 3' untranslated regions (3' UTRs). The location of poly(A) sites with respect to the translational termination codon varies considerably among genes, but whether this has any functional significance is poorly understood. Using hierarchical clustering of 3' UTRs, we identify eight classes of S. cerevisiae genes based on their poly(A) site locations. Genes involved in related biological functions (GO categories) are uniquely over-represented in six of these classes. Similar analysis of S. pombe genes reveals three classes of 3' UTRs, all of which show over-representation of functionally related genes. Remarkably, S. cerevisiae and S. pombe homologs share related patterns of poly(A) site locations. These observations suggest that the location of poly(A) sites within 3' UTRs has biological significance.

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多腺苷酸化位点在 3' 非翻译区内的位置与酵母的生物功能有关。

一个典型的酵母基因表达后会产生 50 个 3' mRNA 异构体，这些异构体是根据 3' 非翻译区（3' UTR）中多聚（A）位点的位置来区分的。多聚（A）位点相对于翻译终止密码子的位置在不同基因之间有很大差异，但这是否具有任何功能意义却鲜为人知。通过对 3' UTR 进行分层聚类，我们根据多聚（A）位点的位置确定了八类 S. cerevisiae 基因。参与相关生物功能（GO 类别）的基因在其中六类基因中具有独特的高代表性。对 S. pombe 基因的类似分析显示，3' UTR 有三个类别，所有这些类别都显示出功能相关基因的过度代表性。值得注意的是，S. cerevisiae 和 S. pombe 同源物的 poly(A) 位点位置具有相关的模式。这些观察结果表明，3' UTR 中 poly(A) 位点的位置具有生物学意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics GENETICS & HEREDITY-

CiteScore

6.90

自引率

6.10%

发文量

177

审稿时长

1.5 months

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.