The effect of age on aqueous humor of humans with high myopia.

IF 1.8 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Vision Pub Date : 2024-03-20 eCollection Date: 2024-01-01

Kai Wen, Mengjun Fu, Yongtao Li, Haorun Zhang, Xiu Wang, Yang Cai, Yaoling Li, Ruihong Su, Yifang Huang, Ming Liu, Yufeng Zhang, Shaozhen Zhao, Jing Sun

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引用次数: 0

Abstract

Background: High myopia is a common cause of vision loss. Age is an important factor in the development of high myopia. However, the effect of age on aqueous humor proteins in the context of high myopia is unknown. This study explored the effect of age on the aqueous humor protein of humans with high myopia.

Methods: The aqueous humor of high myopia patients of different ages with implantable collamer lens implantation (ICL) was collected. Data-independent acquisition proteomic analysis was employed to explore differentially expressed proteins (DEPs). Two different bioinformatics analysis methods were used to interpret the proteomic results. Furthermore, three proteins were confirmed by enzyme-linked immunosorbent assay (ELISA).

Results: The study showed 18 upregulated and 20 downregulated proteins. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the upregulated DEPs were highly enriched in coagulation and complement cascades. Weighted gene coexpression network analysis showed that the blue module was identified as a key module for high myopia and that the plasminogen (PLG) protein is a hub protein. ELISA confirmed that the expression levels of Alpha-1-antitrypsin were signiﬁcantly upregulated in the aqueous humor of older patients presenting with high myopia.

Conclusions: This is the first study to investigate the effect of age on the level of aqueous humor protein in high myopia. Our study provided a comprehensive data set on the overall protein changes of different ages of human high myopia, shedding light on its potential molecular mechanism in high myopia damage to the eyeball.

本刊更多论文

年龄对高度近视患者水样物质的影响。

背景：高度近视是导致视力下降的常见原因。年龄是导致高度近视的一个重要因素。然而，年龄对高度近视患者房水蛋白的影响尚不清楚。本研究探讨了年龄对高度近视患者房水蛋白的影响：方法：研究人员收集了不同年龄段的高度近视患者的房水，这些患者都接受了可植入性准分子人工晶体植入术（ICL）。方法：收集了不同年龄段植入可植入型准分子晶体（ICL）的高度近视患者的水样物质，并采用数据无关的采集蛋白质组分析来探索差异表达蛋白质（DEPs）。使用两种不同的生物信息学分析方法来解释蛋白质组学结果。此外，还通过酶联免疫吸附试验（ELISA）确认了三种蛋白质：结果：研究发现了 18 种上调蛋白和 20 种下调蛋白。京都基因和基因组百科全书（KEGG）分析表明，上调的 DEPs 高度富集于凝血和补体级联。加权基因共表达网络分析显示，蓝色模块被确定为高度近视的关键模块，而纤溶酶原（PLG）蛋白是一个枢纽蛋白。酶联免疫吸附试验证实，在老年高度近视患者的房水中，α-1-抗胰蛋白酶的表达水平显著上调：这是首次研究年龄对高度近视患者房水蛋白水平的影响。我们的研究提供了人类高度近视不同年龄段蛋白质整体变化的全面数据，揭示了高度近视对眼球损伤的潜在分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.