Characterization of sub-micrometre-sized voids in fixed human brain tissue using scanning X-ray microdiffraction.

IF 6.1 3区 材料科学 Q1 Biochemistry, Genetics and Molecular Biology
Prakash Nepal, Abdullah A Bashit, Lee Makowski
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引用次数: 0

Abstract

Using a 5 µm-diameter X-ray beam, we collected scanning X-ray microdiffraction in both the small-angle (SAXS) and the wide-angle (WAXS) regimes from thin sections of fixed human brain tissue from Alzheimer's subjects. The intensity of scattering in the SAXS regime of these patterns exhibits essentially no correlation with the observed intensity in the WAXS regime, indicating that the structures responsible for these two portions of the diffraction patterns, which reflect different length scales, are distinct. SAXS scattering exhibits a power-law behavior in which the log of intensity decreases linearly with the log of the scattering angle. The slope of the log-log curve is roughly proportional to the intensity in the SAXS regime and, surprisingly, inversely proportional to the intensity in the WAXS regime. We interpret these observations as being due to the presence of sub-micrometre-sized voids formed during dehydration of the fixed tissue. The SAXS intensity is due largely to scattering from these voids, while the WAXS intensity derives from the secondary structures of macromolecular material surrounding the voids. The ability to detect and map the presence of voids within thin sections of fixed tissue has the potential to provide novel information on the degradation of human brain tissue in neurodegenerative diseases.

利用扫描 X 射线微衍射技术确定固定人体脑组织中亚微米级空隙的特征。
我们使用直径为 5 µm 的 X 射线束,从固定的阿尔茨海默氏症受试者人脑组织薄片上收集了小角度(SAXS)和广角(WAXS)扫描 X 射线微衍射。这些衍射图样在 SAXS 系统中的散射强度与在 WAXS 系统中观察到的强度基本没有相关性,这表明衍射图样的这两部分反映了不同的长度尺度,其结构是截然不同的。SAXS 散射表现出一种幂律行为,即强度的对数随散射角的对数线性下降。对数-对数曲线的斜率与 SAXS 阶段的强度大致成正比,而令人惊讶的是,与 WAXS 阶段的强度成反比。我们将这些观察结果解释为固定组织脱水过程中形成的亚微米级空隙的存在。SAXS 强度主要来自这些空隙的散射,而 WAXS 强度则来自空隙周围大分子材料的二级结构。检测和绘制固定组织薄切片中空洞的能力有可能为神经退行性疾病中人类脑组织的退化提供新的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
10.00
自引率
3.30%
发文量
178
审稿时长
4.7 months
期刊介绍: Many research topics in condensed matter research, materials science and the life sciences make use of crystallographic methods to study crystalline and non-crystalline matter with neutrons, X-rays and electrons. Articles published in the Journal of Applied Crystallography focus on these methods and their use in identifying structural and diffusion-controlled phase transformations, structure-property relationships, structural changes of defects, interfaces and surfaces, etc. Developments of instrumentation and crystallographic apparatus, theory and interpretation, numerical analysis and other related subjects are also covered. The journal is the primary place where crystallographic computer program information is published.
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