Long-Term Follow-up After Ovariectomy Reveals Correlations Between Bone Marrow Adiposity and Trabecular Bone Quality in the Proximal Metaphysis of Tibiae in Rats.

IF 3.3 3区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Calcified Tissue International Pub Date : 2024-11-01 Epub Date: 2024-10-07 DOI:10.1007/s00223-024-01298-x
Maxime Bedez, Guillaume Falgayrac, Hélène Béhal, Émeline Cailliau, Jérôme Delattre, Xavier Coutel, Cécile Olejnik
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引用次数: 0

Abstract

This study aimed to evaluate the correlation between BMAT and bone quality, describe the long-term effects of ovariectomy on bone, and investigate BMAT's spatial distribution. Fifteen-months-old female Sprague‒Dawley rats were studied, comparing ovariectomized (OVX, n = 22) and sham-operated (SHAM, n = 11) groups at 6 months. Tibias were analyzed for bone microarchitecture, BMAT (microcomputed tomography), mineral parameters (quantitative backscattered electron imaging), and bone composition (Raman microspectroscopy). The OVX tibias showed severe trabecular bone loss (lower bone volume/total volume, p < 0.001) with increased BMAT (higher adipose volume per marrow volume, p < 0.001), decreased mineral content (lower calcium concentration, p < 0.001), and altered organic components (lower mineral/matrix ratio in new bone, p = 0.03 trabecular surface, p < 0.001 trabecular core). When the data are pooled over both groups (SHAM and OVX), the adipose volume/marrow volume ratio was negatively correlated with bone volume/total volume (r =  - 0.79, p < 0.001) and mineral/matrix ratio (r =  - 0.37, p = 0.04 trabecular surface; r =  - 0.65, p < 0.001 trabecular core) and positively correlated with crystallinity (r = 0.55, p = 0.001 trabecular surface; r = 0.49, p = 0.006 trabecular core). The mineral/matrix ratio of trabecular surface new bone was strongly negatively correlated with the adipose compartment nearest to the bone surface. These findings suggest mechanisms underlying BMAT's role in bone resorption.

卵巢切除术后的长期随访揭示了大鼠胫骨近端干骺端骨髓脂肪含量与骨小梁质量之间的相关性
本研究旨在评估 BMAT 与骨质之间的相关性,描述卵巢切除术对骨骼的长期影响,并研究 BMAT 的空间分布。研究对象为 15 个月大的雌性 Sprague-Dawley 大鼠,比较卵巢切除组(OVX,n = 22)和假手术组(SHAM,n = 11)在 6 个月时的情况。对胫骨的骨微结构、BMAT(微型计算机断层扫描)、矿物质参数(定量反向散射电子成像)和骨成分(拉曼微光谱)进行了分析。OVX胫骨显示出严重的骨小梁流失(骨量/总骨量降低,p
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来源期刊
Calcified Tissue International
Calcified Tissue International 医学-内分泌学与代谢
CiteScore
8.00
自引率
2.40%
发文量
112
审稿时长
4-8 weeks
期刊介绍: Calcified Tissue International and Musculoskeletal Research publishes original research and reviews concerning the structure and function of bone, and other musculoskeletal tissues in living organisms and clinical studies of musculoskeletal disease. It includes studies of cell biology, molecular biology, intracellular signalling, and physiology, as well as research into the hormones, cytokines and other mediators that influence the musculoskeletal system. The journal also publishes clinical studies of relevance to bone disease, mineral metabolism, muscle function, and musculoskeletal interactions.
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