Genotypic variation in winter wheat for fusarium foot rot and its biocontrol using Clonostachys rosea.

IF 2.1 3区 生物学 Q3 GENETICS & HEREDITY
Sidhant Chaudhary, Rosa Margarida Nogueira Ricardo, Mukesh Dubey, Dan Funck Jensen, Laura Grenville-Briggs, Magnus Karlsson
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Abstract

Biological control to manage plant diseases is an environmentally friendly alternative to using chemical pesticides. However, little is known about the role of genetic variation in plants affecting the efficacy of biological control agents (BCAs). The aim of this study was to explore the genetic variation in winter wheat for disease susceptibility to fusarium foot rot caused by Fusarium graminearum and variation in biocontrol efficacy of the fungal BCA Clonostachys rosea to control the disease. In total, 190 winter wheat genotypes were evaluated under controlled conditions in two treatments, i.e. (i) F. graminearum (Fg) and (ii) F. graminearum infection on C. rosea treated seeds (FgCr). Alongside disease severity, plant growth-related traits such as shoot length and root length were also measured. Comparison of genotypes between the two treatments enabled the dissection of genotypic variation for disease resistance and C. rosea efficacy. The study revealed significant variation among plant genotypes for fusarium foot rot susceptibility and other growth traits in treatment Fg. Moreover, significant variation in C. rosea efficacy was also observed in genotype contrasts between the two treatments for all traits. Using a 20K marker array, a genome-wide association study was also performed. We identified a total of 18 significant marker-trait associations for disease resistance and C. rosea efficacy for all the traits. Moreover, the markers associated with disease resistance and C. rosea efficacy were not co-localized, highlighting the independent inheritance of these traits, which can facilitate simultaneous selection for cultivar improvement.

冬小麦镰刀菌足腐病的基因型变异及其利用 Clonostachys rosea 进行的生物防治。
生物防治植物病害是使用化学农药的一种环保型替代方法。然而,人们对植物遗传变异影响生物防治剂(BCA)功效的作用知之甚少。本研究旨在探索冬小麦对禾谷镰刀菌引起的镰刀菌足腐病的易感性的遗传变异,以及真菌 BCA Clonostachys rosea 对该病的生物防治效果的变异。在两种处理(即(i) 禾谷镰刀菌(Fg)和(ii) 经 C. rosea 处理的种子上的禾谷镰刀菌感染(FgCr))的对照条件下,共对 190 种冬小麦基因型进行了评估。除病害严重程度外,还测量了植物生长相关性状,如芽长和根长。通过比较两种处理之间的基因型,可以分析抗病性和 C. rosea 药效的基因型变异。研究发现,在处理 Fg 中,植物基因型对镰刀菌足腐病的敏感性和其他生长性状存在显著差异。此外,在两个处理之间的基因型对比中,也观察到玫瑰茄药效在所有性状上的显著差异。我们还利用 20K 标记阵列进行了全基因组关联研究。在所有性状中,我们共发现了 18 个与抗病性和玫瑰果药效相关的重要标记。此外,与抗病性和玫瑰茄药效相关的标记并不共定位,这突出表明了这些性状的独立遗传性,有助于同时进行品种改良选择。
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来源期刊
G3: Genes|Genomes|Genetics
G3: Genes|Genomes|Genetics GENETICS & HEREDITY-
CiteScore
5.10
自引率
3.80%
发文量
305
审稿时长
3-8 weeks
期刊介绍: G3: Genes, Genomes, Genetics provides a forum for the publication of high‐quality foundational research, particularly research that generates useful genetic and genomic information such as genome maps, single gene studies, genome‐wide association and QTL studies, as well as genome reports, mutant screens, and advances in methods and technology. The Editorial Board of G3 believes that rapid dissemination of these data is the necessary foundation for analysis that leads to mechanistic insights. G3, published by the Genetics Society of America, meets the critical and growing need of the genetics community for rapid review and publication of important results in all areas of genetics. G3 offers the opportunity to publish the puzzling finding or to present unpublished results that may not have been submitted for review and publication due to a perceived lack of a potential high-impact finding. G3 has earned the DOAJ Seal, which is a mark of certification for open access journals, awarded by DOAJ to journals that achieve a high level of openness, adhere to Best Practice and high publishing standards.
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