The Optejet Technology Minimizes Preservative-Mediated Cytotoxicity of Conjunctival Epithelial Cells Treated with Latanoprost In Vitro.

IF 1.9 4区 医学 Q2 OPHTHALMOLOGY
Ayesha Sultan, Deshea L Harris, Peter Lam, Julie Whitcomb, Pedram Hamrah
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Abstract

Purpose: Benzalkonium chloride (BAK) is a commonly used preservative to maintain sterility for multiuse eye drops such as latanoprost. One option to minimize the deleterious effects of BAK in eye drops may be to reduce the volume administered. The aim of this study was to assess the response of cells from the ocular surface to latanoprost+BAK administered by the Optejet technology, which dispenses a microdose (∼8 µL) ophthalmical spray. Methods: Cultured human conjunctival epithelial cells were exposed to the following treatments: (1) no treatment, (2) drop form of latanoprost without BAK (∼35 µL), (3) drop form of latanoprost with 0.01% BAK (∼35 µL), (4) ophthalmical spray form of latanoprost with 0.01% BAK delivered by the Optejet technology (∼8 µL). After 5 h, cells were assessed for changes in cytotoxicity, morphology, and inflammatory marker expression. Results: Latanoprost+BAK delivered by a drop induced cytotoxicity, cytoplasmic shrinkage, and loss of cell-cell contact, and expression of chemokine (C-C motif) ligand 2 and interleukin-6. In contrast, latanoprost+BAK delivered by the Optejet technology was both well tolerated and similar to no treatment controls and BAK-free latanoprost treatment. Conclusions: A microdose of latanoprost+BAK ophthalmical spray administered with the Optejet technology prevented the cytotoxicity associated with larger volumes found in eye drops. Precision dosing by the Optejet technology has the potential to decrease ocular surface disorder typically associated with eye drops containing preservatives.

Optejet 技术最大程度地减少了拉坦前列腺体外处理结膜上皮细胞时防腐剂引起的细胞毒性。
目的:苯扎氯铵(BAK)是一种常用的防腐剂,用于保持拉坦前列素等多用途滴眼液的无菌性。减少滴眼液中 BAK 的有害影响的一种方法是减少用量。本研究的目的是评估眼表细胞对使用Optejet技术滴入的拉坦前列素+BAK的反应。方法将培养的人类结膜上皮细胞暴露于以下处理中:(1)不处理;(2)不含BAK的拉坦前列素滴剂(∼35 µL);(3)含0.01% BAK的拉坦前列素滴剂(∼35 µL);(4)通过Optejet技术递送的含0.01% BAK的拉坦前列素眼科喷雾剂(∼8 µL)。5 小时后,评估细胞毒性、形态和炎症标志物表达的变化。结果:滴注拉坦前列腺素+BAK可诱导细胞毒性、细胞质萎缩、细胞-细胞接触丧失以及趋化因子(C-C motif)配体2和白细胞介素-6的表达。相比之下,通过 Optejet 技术输送的拉坦前列腺素+BAK 具有良好的耐受性,与无治疗对照组和不含 BAK 的拉坦前列腺素治疗相似。结论采用Optejet技术的微剂量拉坦前列素+BAK眼用喷雾剂避免了滴眼液中较大剂量所产生的细胞毒性。Optejet 技术的精确剂量有可能减少通常与含有防腐剂的眼药水相关的眼表紊乱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.60
自引率
4.30%
发文量
72
审稿时长
1 months
期刊介绍: Journal of Ocular Pharmacology and Therapeutics is the only peer-reviewed journal that combines the fields of ophthalmology and pharmacology to enable optimal treatment and prevention of ocular diseases and disorders. The Journal delivers the latest discoveries in the pharmacokinetics and pharmacodynamics of therapeutics for the treatment of ophthalmic disorders. Journal of Ocular Pharmacology and Therapeutics coverage includes: Glaucoma Cataracts Retinal degeneration Ocular infection, trauma, and toxicology Ocular drug delivery and biotransformation Ocular pharmacotherapy/clinical trials Ocular inflammatory and immune disorders Gene and cell-based therapies Ocular metabolic disorders Ocular ischemia and blood flow Proliferative disorders of the eye Eyes on Drug Discovery - written by Gary D. Novack, PhD, featuring the latest updates on drug and device pipeline developments as well as policy/regulatory changes by the FDA.
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