Enhancement of apoptosis in Caco-2, Hep-G2, and HT29 cancer cell lines following exposure to Toxoplasma gondii peptides.

IF 2 Q3 PHARMACOLOGY & PHARMACY
Drug Target Insights Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI:10.33393/dti.2024.3177
Firooz Shahrivar, Javid Sadraei, Majid Pirestani, Ehsan Ahmadpour
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引用次数: 0

Abstract

Objective: Cancer or neoplasm is a cosmopolitan catastrophe that results in more than 20 million new cases and 10 million deaths every year. Some factors lead to carcinogenesis like infectious diseases. Parasites like Toxoplasma gondii, by its components, could modulate the cancer system by inducing apoptosis. The objective of this investigation is to assess the potential of peptides derived from T. gondii in combating cancer by examining their effects on Caco-2, Hep-G2, and HT29 cell lines.

Materials and methods: Candidate peptide by its similarity to anticancer compounds was predicted through the computer-based analysis/platform. The impact of the peptide on cell viability, cell proliferation, and gene expression was evaluated through the utilization of MTT assay, flow cytometry, and real-time polymerase chain reaction (PCR) methodologies.

Results: The cell viability rate exhibited a significant decrease (p < 0.001) across all cell lines when exposed to a concentration of ≤160 μg. Within the 48-hour timeframe, the half maximal inhibitory concentration (IC50) for HT29 and Hep-G2 cell lines was determined to be 107.2 and 140.6 μg/mL, respectively. Notably, a marked decrease in the expression levels of Bcl2 and APAF1 genes was observed in both the Hep-G2 and HT29 cell lines.

Conclusion: These findings indicate that the T. gondii peptide affected cancer cell mortality and led to changes in the expression of genes associated with apoptosis.

接触弓形虫肽后,Caco-2、Hep-G2 和 HT29 癌细胞系的细胞凋亡增强。
目的:癌症或肿瘤是一种世界性灾难,每年导致 2 000 多万新发病例和 1 000 多万人死亡。一些因素会导致癌症的发生,如传染病。弓形虫等寄生虫的成分可通过诱导细胞凋亡来调节癌症系统。本研究的目的是通过检测弓形虫肽对 Caco-2、Hep-G2 和 HT29 细胞系的影响,评估其抗癌潜力:通过计算机分析/平台预测与抗癌化合物相似的候选肽。通过 MTT 检测法、流式细胞仪和实时聚合酶链反应(PCR)方法评估了多肽对细胞活力、细胞增殖和基因表达的影响:结果:当暴露于浓度≤160 μg的肽时,所有细胞系的细胞存活率都明显下降(p < 0.001)。在 48 小时内,HT29 和 Hep-G2 细胞系的半数最大抑制浓度(IC50)分别为 107.2 和 140.6 μg/mL。值得注意的是,在 Hep-G2 和 HT29 细胞系中都观察到 Bcl2 和 APAF1 基因的表达水平明显下降:结论:这些研究结果表明,淋球菌肽会影响癌细胞的死亡率,并导致与细胞凋亡相关的基因表达发生变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Drug Target Insights
Drug Target Insights PHARMACOLOGY & PHARMACY-
CiteScore
2.70
自引率
0.00%
发文量
5
审稿时长
8 weeks
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