Dynamics of glutamine synthetase expression in hepatic ischemia-reperfusion injury: Implications for therapeutic interventions.

IF 2.5 Q2 GASTROENTEROLOGY & HEPATOLOGY

World Journal of Hepatology Pub Date : 2024-08-27 DOI:10.4254/wjh.v16.i8.1177

Zhi-Hao Huang, Meng-Qi Dong, Feng-Yong Liu, Wei-Jie Zhou

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Abstract

Background: Hepatic ischemia-reperfusion injury (IRI) poses a great challenge in liver surgery and transplantation because of oxidative stress and inflammatory responses. The changes in glutamine synthetase (GS) expression during hepatic IRI remain unclear.

Aim: To investigate the dynamic expression of GS during hepatic IRI.

Methods: Following hepatic ischemia for 1 h and reperfusion, liver tissue samples were collected at 0.5, 6, and 24 hours postreperfusion for fixation, embedding, sectioning. Hematoxylin and eosin staining and GS staining were performed.

Results: GS expression rapidly decreases in hepatocytes around the central vein after IRI, reaching its lowest point at 6 hours postreperfusion, and then gradually recovers.

Conclusion: GS is highly sensitive to IRI, highlighting its potential role as an indicator of liver injury states and a target for therapeutic intervention.

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肝缺血再灌注损伤中谷氨酰胺合成酶的表达动态：治疗干预的意义。

背景：由于氧化应激和炎症反应，肝缺血再灌注损伤（IRI）对肝脏手术和移植构成了巨大挑战。目的：研究肝脏缺血再灌注损伤过程中谷氨酰胺合成酶（GS）的动态表达：方法：肝脏缺血 1 小时并再灌注后，在再灌注后 0.5、6 和 24 小时采集肝组织样本，进行固定、包埋和切片。结果表明：在肝细胞再灌注后 0.5、6 和 24 小时，GS 表达迅速下降：结果：IRI后中央静脉周围肝细胞的GS表达迅速下降，在再灌注后6小时达到最低点，然后逐渐恢复：结论：GS对IRI高度敏感，突显了其作为肝损伤状态指标和治疗干预目标的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World Journal of Hepatology GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

4.10

自引率

4.20%

发文量

172