Electroacupuncture Promotes the Generation of Intestinal Treg Cells After Ischemic Stroke by Foxp3 Acetylation Regulation.

IF 4.6 2区医学 Q1 NEUROSCIENCES

Molecular Neurobiology Pub Date : 2025-03-01 Epub Date: 2024-09-25 DOI:10.1007/s12035-024-04500-1

Yonglin Chen, Ling Ouyang, Xinyi Yang, Bufan Wu, Lingling Meng, Jialin Gu, Yaling Wang, Juan Li, Jingjing Zhang, Xinyue Jing, Shengfeng Lu, Lanying Liu, Shuping Fu

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引用次数: 0

Abstract

Electroacupuncture (EA) has been shown to ameliorate brain injury and protect against intestinal injury after ischemic stroke. These protective effects are closely associated with the enhancement of regulatory T (Treg) cell numbers and function in the intestine, as well as the inhibition of intestinal γδ T cell production and their migration to the brain. This study aimed to elucidate the potential mechanism by which EA regulates intestinal Treg cell differentiation after stroke. Sprague-Dawley rats were divided into three groups: the sham group, the middle cerebral artery occlusion (MCAO) group, and the MCAO plus EA (MEA) group. The MCAO model was generated by occluding the middle cerebral artery. EA was applied to Baihui (GV20) acupoint once daily. Samples were collected 3 days after reperfusion. Our results showed that EA reduced the inflammatory response in the brain and intestine after ischemic stroke. EA treatment increased the percentage of Treg cells in the small intestine of rats. EA increased the levels of SCFAs, while also inhibiting histone deacetylase activity (HDAC). Additionally, acetylated Foxp3 protein in the small intestine was increased after EA treatment. These results suggest that EA at GV20 alleviates brain and intestinal inflammatory injury in stroke rats, potentially through the enhancement of SCFA-mediated Foxp3 acetylation in Treg cells.

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电针通过Foxp3乙酰化调控促进缺血性脑卒中后肠道Treg细胞的生成

电针（EA）已被证明可改善缺血性中风后的脑损伤并保护肠道免受损伤。这些保护作用与增强肠道调节性 T（Treg）细胞的数量和功能、抑制肠道 γδ T 细胞的产生及其向大脑的迁移密切相关。本研究旨在阐明 EA 调节脑卒中后肠道 Treg 细胞分化的潜在机制。研究将 Sprague-Dawley 大鼠分为三组：假组、大脑中动脉闭塞（MCAO）组和 MCAO 加 EA（MEA）组。MCAO 模型通过闭塞大脑中动脉产生。每天一次在百会穴（GV20）上注射 EA。再灌注3天后收集样本。结果表明，EA能减轻缺血性中风后大脑和肠道的炎症反应。EA治疗增加了大鼠小肠中Treg细胞的比例。EA 增加了 SCFAs 的水平，同时还抑制了组蛋白去乙酰化酶（HDAC）的活性。此外，EA 处理后小肠中乙酰化的 Foxp3 蛋白增加。这些结果表明，GV20 的 EA 可减轻中风大鼠的脑和肠道炎症损伤，这可能是通过增强 Treg 细胞中 SCFA 介导的 Foxp3 乙酰化作用实现的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Neurobiology 医学-神经科学

CiteScore

9.00

自引率

2.00%

发文量

480

审稿时长

1 months

期刊介绍： Molecular Neurobiology is an exciting journal for neuroscientists needing to stay in close touch with progress at the forefront of molecular brain research today. It is an especially important periodical for graduate students and "postdocs," specifically designed to synthesize and critically assess research trends for all neuroscientists hoping to stay active at the cutting edge of this dramatically developing area. This journal has proven to be crucial in departmental libraries, serving as essential reading for every committed neuroscientist who is striving to keep abreast of all rapid developments in a forefront field. Most recent significant advances in experimental and clinical neuroscience have been occurring at the molecular level. Until now, there has been no journal devoted to looking closely at this fragmented literature in a critical, coherent fashion. Each submission is thoroughly analyzed by scientists and clinicians internationally renowned for their special competence in the areas treated.