Effects of multisuperovulation on the transcription and genomic methylation of oocytes and offspring.

IF 4.8 2区医学 Q1 GENETICS & HEREDITY

Clinical Epigenetics Pub Date : 2024-09-28 DOI:10.1186/s13148-024-01746-3

Juan-Ke Xie, Qian Wang, Yuan-Hui Chen, Shou-Bin Tang, Hao-Yue Sun, Zhao-Jia Ge, Cui-Lian Zhang

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引用次数: 0

Abstract

Background: Controlled ovarian stimulation is a common skill of assisted reproductive technologies (ARTs). In the clinic, some females would undergo more than one controlled ovarian stimulation cycle. However, few studies have focused on the influence of multi-superovulation on oocytes and offspring.

Results: Here, we found that multi-superovulation disrupted the transcriptome of oocytes and that the differentially expressed genes (DEGs) were associated mainly with metabolism and fertilization. The disruption of mRNA degradation via poly (A) size and metabolism might be a reason for the reduced oocyte maturation rate induced by repeated superovulation. Multi-superovulation results in hypo-genomic methylation in oocytes. However, there was an increase in the methylation level of CGIs. The DMRs are not randomly distributed in genome elements. Genes with differentially methylated regions (DMRs) in promoters are enriched in metabolic pathways. With increasing of superovulation cycles, the glucose and insulin tolerance of offspring is also disturbed.

Conclusions: These results suggest that multi-superovulation has adverse effects on oocyte quality and offspring health.

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多次超排卵对卵母细胞及后代转录和基因组甲基化的影响

背景：控制性卵巢刺激是辅助生殖技术（ART）的一项常用技能。在临床中，一些女性会接受一个以上的控制性卵巢刺激周期。然而，很少有研究关注多次超排卵对卵母细胞和后代的影响：结果：我们发现，多次超级排卵破坏了卵母细胞的转录组，差异表达基因（DEGs）主要与新陈代谢和受精有关。mRNA通过poly (A)大小和新陈代谢降解的中断可能是重复超级排卵诱导卵母细胞成熟率降低的一个原因。多次超排卵会导致卵母细胞基因组甲基化过低。不过，CGIs 的甲基化水平有所提高。DMRs 在基因组元素中并非随机分布。启动子中存在差异甲基化区域（DMRs）的基因富集在代谢途径中。随着超排卵周期的增加，后代的葡萄糖和胰岛素耐受性也会受到干扰：这些结果表明，多次超排卵会对卵母细胞质量和后代健康产生不利影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Epigenetics ONCOLOGY-

自引率

5.30%

发文量

150

期刊介绍： Clinical Epigenetics, the official journal of the Clinical Epigenetics Society, is an open access, peer-reviewed journal that encompasses all aspects of epigenetic principles and mechanisms in relation to human disease, diagnosis and therapy. Clinical trials and research in disease model organisms are particularly welcome.