Nanosensor based on HP-MAP1 and carbon nanotubes for bacteria detection.

IF 2.5 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Progress Pub Date : 2024-09-26 DOI:10.1002/btpr.3510

Winne F S M Silva, Ludovico Migliolo, Patrícia S Silva, Glaucia M S Lima, Maria D L Oliveira, Cesar A S Andrade

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引用次数: 0

Abstract

Healthcare-associated infections (HAIs) pose significant challenges to global health due to pathogen complexity and antimicrobial resistance. Biosensors utilizing antimicrobial peptides offer innovative solutions. Hylarana picturata Multiple Active Peptide 1 (Hp-MAP1), derived from Temporin-PTA, exhibits antibacterial properties sourced from the skin secretions of the Malaysian fire-bellied frog. An innovative sensing layer was developed for the electrochemical biorecognition of diverse pathogens: Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus. Electrochemical impedance spectroscopy differentiated microorganisms based on distinct electrochemical responses. The sensor layer, composed of functionalized multi-walled carbon nanotubes (MWCNTs) associated with Hp-MAP1, exhibited varying levels of charge transfer resistance (R_CT) for different microorganisms. Gram-negative species, especially P. aeruginosa, displayed higher R_CT values, indicating better impedimetric responses. Excellent LODs were observed for P. aeruginosa (0.60), K. pneumoniae (0.42), E. coli (0.67), and S. aureus (0.59), highlighting the efficacy of the MWCNTs/Hp-MAP1 biosensor in microbial identification. The MWCNTs/Hp-MAP1 biosensor platform presents a promising and effective microbial identification strategy with potential healthcare applications to mitigate HAIs and enhance patient care.

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基于 HP-MAP1 和碳纳米管的细菌检测纳米传感器

由于病原体的复杂性和抗菌药的耐药性，医疗相关感染（HAIs）给全球健康带来了重大挑战。利用抗菌肽的生物传感器提供了创新的解决方案。从 Temporin-PTA 中提取的 Hylarana picturata 多活性肽 1 (Hp-MAP1)具有抗菌特性，其来源是马来西亚火腹蛙的皮肤分泌物。为电化学生物识别各种病原体开发了一种创新的传感层：铜绿假单胞菌、肺炎克雷伯氏菌、大肠杆菌和金黄色葡萄球菌。电化学阻抗光谱根据不同的电化学反应区分微生物。传感器层由与 Hp-MAP1 相关的功能化多壁碳纳米管（MWCNTs）组成，对不同微生物表现出不同程度的电荷转移电阻（RCT）。革兰氏阴性菌，尤其是铜绿假单胞菌，显示出更高的 RCT 值，表明阻抗响应更好。对于铜绿假单胞菌（0.60）、肺炎双球菌（0.42）、大肠杆菌（0.67）和金黄色葡萄球菌（0.59），观察到了极佳的 LOD 值，这凸显了 MWCNTs/Hp-MAP1 生物传感器在微生物鉴定方面的功效。MWCNTs/Hp-MAP1 生物传感器平台是一种前景广阔的有效微生物鉴定策略，具有潜在的医疗保健应用价值，可减轻 HAIs 并加强病人护理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Progress 工程技术-生物工程与应用微生物

CiteScore

6.50

自引率

3.40%

发文量

审稿时长

4 months

期刊介绍： Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.