Sparing Effect on Cell Survival Under Normoxia Using Ultra-high Dose Rate Proton Beams from a Compact Superconducting AVF Cyclotron.

IF 1.6 4区医学 Q4 ONCOLOGY

Anticancer research Pub Date : 2024-10-01 DOI:10.21873/anticanres.17255

Masashi Yagi, Kazumasa Minami, Kazuki Fujita, Shinji Nomura, Nagaaki Kamiguchi, Kana Nagata, Ryo Hidani, Daizo Amano, Kenzo Sasai, Shinichi Shimizu, Kazuhiko Ogawa

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引用次数: 0

Abstract

Background/aim: The purpose of this study was to evaluate whether the sparing effect on cell survival is observed under normoxia.

Materials and methods: A superconducting spiral sector-type azimuthally varying field (AVF) cyclotron produced 230 MeV proton beams at 250 Gy/s as ultra-high dose rate (uHDR) and 1 Gy/s as normal dose rate (NDR) to irradiate tumor and normal cell lines (HSGc-c5 and HDF up to 24 Gy at the center of spread-out Bragg peak (SOBP). The Advanced Markus chamber and Gafchromic film were used to measure the examined absolute dose and field sizes. Colony formation assay and immunofluorescence staining were conducted to evaluate the sparing effect.

Results: A homogeneous field was achieved at the center of the SOBP for both uHDR and NDR scanned proton beams, and dose reproducibility and linearity were adequate for experiments. There were significant differences in cell surviving fractions of HSGc-C5 and HDF cells irradiated at uHDRs compared to NDRs at 20 Gy and 24 Gy. Increasing γ-H2AX foci were observed for both cell lines at NDR.

Conclusion: The sparing effect on cell survival was first observed under normoxic conditions for tumor and normal cells with doses exceeding 20 Gy, using proton irradiation at 250 Gy/s extracted from a superconducting AVF cyclotron. This study marks a significant milestone in advancing our understanding of the underlying mechanism behind the sparing effect.

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利用紧凑型超导 AVF 回旋加速器产生的超高剂量率质子束在常氧条件下对细胞存活的疏导作用

背景/目的：本研究的目的是评估在常氧条件下是否能观察到对细胞存活的抑制作用：超导螺旋扇形方位变化场（AVF）回旋加速器以250 Gy/s的超高剂量率（uHDR）和1 Gy/s的正常剂量率（NDR）产生230 MeV质子束，对肿瘤细胞株和正常细胞株（HSGc-c5和HDF）进行辐照，在扩散布拉格峰（SOBP）中心达到24 Gy。先进的 Markus 室和 Gafchromic 膜用于测量所检查的绝对剂量和场尺寸。进行了菌落形成检测和免疫荧光染色，以评估疏导效果：结果：uHDR 和 NDR 扫描质子束在 SOBP 中心都能获得均匀的射野，剂量的可重复性和线性都能满足实验要求。与 20 Gy 和 24 Gy 的 NDR 相比，uHDR 照射的 HSGc-C5 和 HDF 细胞存活率有明显差异。在NDR下，两种细胞系的γ-H2AX病灶都在增加：利用从超导 AVF 回旋加速器中提取的 250 Gy/s 的质子辐照，在常氧条件下首次观察到肿瘤细胞和正常细胞在剂量超过 20 Gy 时对细胞存活的疏导作用。这项研究是一个重要的里程碑，推动了我们对辐照效应背后机理的了解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anticancer research 医学-肿瘤学

CiteScore

3.70

自引率

10.00%

发文量

566

审稿时长

2 months

期刊介绍： ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.