The application of X-ray fluorescence microscopy and micro-XANES spectroscopy to study neuro-metallomics

IF 3.8 2区化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Inorganic Biochemistry Pub Date : 2024-09-24 DOI:10.1016/j.jinorgbio.2024.112744

Meg Willans , Ashley Hollings , Rhiannon E. Boseley , Thomas Munyard , Gaewyn C. Ellison , Mark J. Hackett

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Abstract

This early career research highlight provides a review of my own research program over the last decade, a time frame that encompasses my transition from postdoctoral fellowships to independent researcher. As an analytical chemist and applied spectroscopist, the central theme of my research program over this time has been protocol development at synchrotron facilities, with the main objective to investigate brain metal homeostasis during both brain health and brain disease. I will begin my review with an overview of brain metal homeostasis, before introducing analytical challenges associated with its study. I will then provide a brief summary of the two main X-ray techniques I have used to study brain metal homeostasis, X-ray fluorescence microscopy (XFM) and X-ray absorption near edge structure spectroscopy (XANES). The review then finishes with a summary of my main research contributions using these two techniques, put in the context of the results from others in the field.

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应用 X 射线荧光显微镜和显微 XANES 光谱研究神经金属组学。

这篇早期职业生涯研究亮点回顾了我自己过去十年的研究计划，这十年是我从博士后研究员转变为独立研究员的十年。作为一名分析化学家和应用光谱学家，这段时间我研究计划的中心主题是同步加速器设施的协议开发，主要目标是研究大脑健康和大脑疾病期间的大脑金属稳态。首先，我将概述脑金属稳态，然后介绍与脑金属稳态研究相关的分析挑战。然后，我将简要介绍我用于研究脑金属稳态的两种主要 X 射线技术：X 射线荧光显微镜 (XFM) 和 X 射线吸收近边缘结构光谱 (XANES)。最后，结合该领域其他学者的研究成果，总结了我使用这两种技术的主要研究成果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Inorganic Biochemistry 生物-生化与分子生物学

CiteScore

7.00

自引率

10.30%

发文量

336

审稿时长

41 days

期刊介绍： The Journal of Inorganic Biochemistry is an established international forum for research in all aspects of Biological Inorganic Chemistry. Original papers of a high scientific level are published in the form of Articles (full length papers), Short Communications, Focused Reviews and Bioinorganic Methods. Topics include: the chemistry, structure and function of metalloenzymes; the interaction of inorganic ions and molecules with proteins and nucleic acids; the synthesis and properties of coordination complexes of biological interest including both structural and functional model systems; the function of metal- containing systems in the regulation of gene expression; the role of metals in medicine; the application of spectroscopic methods to determine the structure of metallobiomolecules; the preparation and characterization of metal-based biomaterials; and related systems. The emphasis of the Journal is on the structure and mechanism of action of metallobiomolecules.