A Dicyanoisophorone-based Fluorescent Turn-on Probe for Rapid Detecting Thiophenol in Aqueous Medium and Living Cell Imaging.

Abstract

A novel colorimetric and fluorescent thiophenol probe based on dicyanoisophorone has been successfully achieved, which has low-cost, easy operation, high selectivity, sensitivity and stability. The chemosensor shows a large Stokes shift and approximately 170 nm when excited at 510 nm. ISO-DiNO₂ could be utilized as "Turn-on" and a naked-eyes chemosensor to detect PhSH, which is accompanied by a distinct color shift from red to dark purple with strong red fluorescence at 365 nm UV-light. Its limit of detection was determined to be 1.15 μM. More importantly, ISO-DiNO₂ can react instantaneously (<10 s) with PhS^-. In addition, ISO-DiNO₂ has been utilized in test paper strips, water sample together with imaging of PhS^- in living Raw264.7 cells, demonstrating that ISO-DiNO₂ has excellent and promising applications.