Regulation of cagA-Helicobacter on gastric PIM2 expression in gastric cancer.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-01-01 DOI:10.3233/CBM-230351

Wenjuan Liang, Haiyan Wang, Lijuan Hong, Wenting Cao, Shuaiyin Chen, Rongguang Zhang

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引用次数: 0

Abstract

Background: The association between infection with cagA-positive H. pylori and an elevated susceptibility to gastric cancer has been firmly established. PIM2 is known to be overexpressed in various types of cancers; however, the specific mechanism by which cagA influences the regulation of PIM2 expression in gastric cancer remains unidentified at present.

Materials and methods: A mutant NCTC11637ΔcagA strain of H. pylori and the eukaryotic expression vector pcDNA-cagA were constructed for evaluating PIM2 expression levels in gastric cancer cells (HGC27, SGC7901, and AG) co-cultured with the NCTC11637 and NCTC11637ΔcagA strain, as well as pcDNA-cagA and the empty vector pcDNA3.1 (+).

Results: Co-culturing gastric cancer cells with NCTC11637 significantly increased PIM2 expression levels (P< 0.001) compared to the negative control group. Additionally, the expression of PIM2 in cells co-cultured with NCTC11637 was higher than that co-cultured with NCTC11637ΔcagA (P< 0.001). Furthermore, successful construction of the eukaryotic expression vector pcDNA-cagA resulted in a significant increase in PIM2 mRNA expression levels after its transfection into gastric cancer cells compared to the control group after 48 hours.

Conclusions: The findings indicate that H. pylori/cagA A could be one of the key factors in regulating PIM2 expression levels, potentially influencing the progression of H. pylori-related Gastric Cancer.

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cagA-Helicobacter 对胃癌中胃 PIM2 表达的调控。

背景：感染 cagA 阳性幽门螺杆菌与胃癌易感性升高之间的关系已被证实。已知 PIM2 在各种癌症中过度表达；然而，目前 cagA 影响胃癌中 PIM2 表达调控的具体机制仍未确定：构建幽门螺杆菌突变株NCTC11637ΔcagA和真核表达载体pcDNA-cagA，用于评估与NCTC11637和NCTC11637ΔcagA株以及pcDNA-cagA和空载体pcDNA3.1（+）共培养的胃癌细胞（HGC27、SGC7901和AG）中PIM2的表达水平：结果：与阴性对照组相比，与 NCTC11637 共培养胃癌细胞可显著提高 PIM2 的表达水平（P< 0.001）。此外，与 NCTC11637 共培养的细胞中 PIM2 的表达高于与 NCTC11637ΔcagA 共培养的细胞（P< 0.001）。此外，成功构建真核表达载体 pcDNA-cagA 后，与对照组相比，转染胃癌细胞 48 小时后 PIM2 mRNA 表达水平显著增加：结论：研究结果表明，幽门螺杆菌/cagA A可能是调节PIM2表达水平的关键因素之一，有可能影响幽门螺杆菌相关胃癌的进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464