Thymol and p-Cymene Protect the Liver by Mitigating Oxidative Stress, Suppressing TNF-α/NF-κB, and Enhancing Nrf2/HO-1 Expression in Immobilized Rats

IF 3.2 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yasaman Peirovy, Masoumeh Asle-Rousta
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引用次数: 0

Abstract

This study aimed to investigate the effects of the monoterpenes thymol and p-cymene on the liver of rats subjected to prolonged immobilization stress and to discover the possible mechanism behind this effect. For 14 consecutive days, the rats were placed in a restrainer for 2.5 h every day to expose them to stress. During the same period, thymol (10 mg/kg, gavage) and p-cymene (50 mg/kg, intraperitoneally) were also administered. Thymol and p-cymene prevented the increase in malondialdehyde levels and the decrease in glutathione content in the liver of rats exposed to chronic immobility. They also increased the activity of the glutathione peroxidase enzyme in the liver of stressed animals, but only thymol could increase the activity of superoxide dismutase. These monoterpenes reduced the expression of pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, and IL-6 and nuclear factor kappa B (NF-κB) in the liver of stressed animals. They increased the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). Thymol and p-cymene greatly prevented the infiltration of inflammatory cells in the liver parenchyma of stressed rats. In conclusion, the study found that thymol and p-cymene have a hepatoprotective effect on immobilized rats, likely exerted by suppressing oxidative stress and inflammation, stimulating Nrf2/HO-1 signaling, and inhibiting the TNF-α/NF-κB pathway.

百里酚和对雏菊烯通过减轻氧化应激、抑制 TNF-α/NF-κB、增强 Nrf2/HO-1 表达来保护固定大鼠的肝脏。
本研究旨在探讨单萜类化合物百里酚和对伞花烃对受到长期固定应激的大鼠肝脏的影响,并探索这种影响背后的可能机制。连续14天,每天将大鼠放在束缚器中2.5小时,使其处于应激状态。在此期间,还对大鼠进行了百里酚(10 毫克/千克,灌胃)和对胸腺嘧啶(50 毫克/千克,腹腔注射)试验。胸腺酚和对伞花烃能防止大鼠肝脏中丙二醛含量的增加和谷胱甘肽含量的减少。它们还能提高受压动物肝脏中谷胱甘肽过氧化物酶的活性,但只有百里酚能提高超氧化物歧化酶的活性。这些单萜烯类化合物降低了应激动物肝脏中促炎细胞因子肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6 和核因子卡巴 B(NF-κB)的表达。它们增加了核因子红细胞2相关因子2(Nrf2)和血红素加氧酶1(HO-1)的表达。胸腺酚和对伞花烃能极大地阻止应激大鼠肝实质中炎症细胞的浸润。总之,研究发现胸腺酚和对伞花烃对固定大鼠具有保肝作用,这可能是通过抑制氧化应激和炎症、刺激 Nrf2/HO-1 信号传导以及抑制 TNF-α/NF-κB 通路来实现的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Chemical Biology & Drug Design
Chemical Biology & Drug Design 医学-生化与分子生物学
CiteScore
5.10
自引率
3.30%
发文量
164
审稿时长
4.4 months
期刊介绍: Chemical Biology & Drug Design is a peer-reviewed scientific journal that is dedicated to the advancement of innovative science, technology and medicine with a focus on the multidisciplinary fields of chemical biology and drug design. It is the aim of Chemical Biology & Drug Design to capture significant research and drug discovery that highlights new concepts, insight and new findings within the scope of chemical biology and drug design.
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