Revisiting the role of the spindle assembly checkpoint in the formation of gross chromosomal rearrangements in Saccharomyces cerevisiae.

IF 3.3 3区生物学 Q2 GENETICS & HEREDITY

Genetics Pub Date : 2024-11-06 DOI:10.1093/genetics/iyae150

Yue Yao, Ziqing Yin, Fernando R Rosas Bringas, Jonathan Boudeman, Daniele Novarina, Michael Chang

引用次数: 0

Abstract

Multiple pathways are known to suppress the formation of gross chromosomal rearrangements (GCRs), which can cause human diseases including cancer. In contrast, much less is known about pathways that promote their formation. The spindle assembly checkpoint (SAC), which ensures the proper separation of chromosomes during mitosis, has been reported to promote GCR, possibly by delaying mitosis to allow GCR-inducing DNA repair to occur. Here, we show that this conclusion is the result of an experimental artifact arising from the synthetic lethality caused by the disruption of the SAC and loss of the CIN8 gene, which is often lost in the genetic assay used to select for GCRs. After correcting for this artifact, we find no role of the SAC in promoting GCR.

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重新审视纺锤体装配检查点在酿酒酵母染色体粗大重排形成过程中的作用

已知有多种途径可抑制染色体大重排（GCR）的形成，而染色体大重排可导致包括癌症在内的人类疾病。相比之下，人们对促进染色体重排形成的途径却知之甚少。纺锤体装配检查点（SAC）可确保染色体在有丝分裂过程中正确分离，有报道称其可能通过延迟有丝分裂以允许GCR诱导的DNA修复发生，从而促进GCR的形成。在这里，我们证明了这一结论是由于 SAC 的破坏和 CIN8 基因的缺失导致的合成致死率所造成的实验假象，而 CIN8 基因在用于选择 GCR 的基因测定中经常会丢失。在纠正了这一假象之后，我们发现 SAC 在促进 GCR 方面没有任何作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics GENETICS & HEREDITY-

CiteScore

6.90

自引率

6.10%

发文量

177

审稿时长

1.5 months

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.