In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays.

4区生物学 Q3 Biochemistry, Genetics and Molecular Biology

Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-06-06 DOI:10.1016/bs.mie.2024.05.012

Peter J Harrison, Jake Chandler, Andrew J Thompson, Timothy D H Bugg

引用次数: 0

Abstract

The article reports methods for the expression and assay of 9-cis-epoxycarotenoid cleavage dioxygenase (NCED), an enzyme involved in the biosynthesis of phytohormone abscisic acid in plants. A method for the preparation of the unstable substrate 9'-cis-neoxanthin from fresh spinach is described. The inhibition of Solanum lycopersicum NCED by a series of aryl hydroxamic acid inhibitors is illustrated, and inhibitors D2 and D4 are assayed against NCED isozymes from Zea mays.

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茄属植物和玉米中的 9-顺式环氧类胡萝卜素二加氧酶（NCED）的体外测定和抑制作用。

文章报告了 9-顺式环氧类胡萝卜素裂解二加氧酶（NCED）的表达和检测方法，该酶参与植物中植物激素脱落酸的生物合成。本文介绍了一种从新鲜菠菜中制备不稳定底物 9'-cis-neoxanthin 的方法。说明了一系列芳基羟肟酸抑制剂对 Solanum lycopersicum NCED 的抑制作用，并对抑制剂 D2 和 D4 针对玉米 NCED 同工酶进行了检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in enzymology 生物-生化研究方法

CiteScore

2.90

自引率

0.00%

发文量

308

审稿时长

3-6 weeks

期刊介绍： The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.