Evaluation of Klebsiella pneumoniae pathogenicity through holistic gene content analysis.

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
Miyu Isogai, Kumiko Kawamura, Tetsuya Yagi, Shizuo Kayama, Motoyuki Sugai, Yohei Doi, Masahiro Suzuki
{"title":"Evaluation of <i>Klebsiella pneumoniae</i> pathogenicity through holistic gene content analysis.","authors":"Miyu Isogai, Kumiko Kawamura, Tetsuya Yagi, Shizuo Kayama, Motoyuki Sugai, Yohei Doi, Masahiro Suzuki","doi":"10.1099/mgen.0.001295","DOIUrl":null,"url":null,"abstract":"<p><p><i>Klebsiella pneumoniae</i> is a Gram-negative bacterium that causes both community- and healthcare-associated infections. Although various virulence factors and highly pathogenic phenotypes have been reported, the pathogenicity of <i>K. pneumoniae</i> is still not fully understood. In this study, we utilized whole-genome sequencing data of 168 clinical <i>K. pneumoniae</i> strains to assess pathogenicity. This work was based on the concept that the genetic composition of individual genomes (referred to as holistic gene content) of the strains may contribute to their pathogenicity. Holistic gene content analysis revealed two distinct groups of <i>K. pneumoniae</i> strains ('major group' and 'minor group'). The minor group included strains with known highly pathogenic clones (ST23, ST375, ST65 and ST86). The minor group had higher rates of capsular genotype K1 and presence of nine specific virulence genes (<i>rmpA</i>, <i>iucA</i>, <i>iutA</i>, <i>irp2</i>, <i>fyuA</i>, <i>ybtS</i>, <i>iroN</i>, <i>allS</i> and <i>clbA</i>) compared to the major group. Pathogenicity was assessed using <i>Galleria mellonella</i> larvae. Infection experiments revealed lower survival rates of larvae infected with strains from the minor group, indicating higher virulence. In addition, the minor group had a higher string test positivity rate than the major group. Holistic gene content analysis predicted possession of virulence genes, string test positivity and pathogenicity as observed in the <i>G. mellonella</i> infection model. Moreover, the findings suggested the presence of as yet unrecognized genomic elements that are either involved in the acquisition of virulence genes or associated with pathogenicity.</p>","PeriodicalId":18487,"journal":{"name":"Microbial Genomics","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial Genomics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/mgen.0.001295","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0

Abstract

Klebsiella pneumoniae is a Gram-negative bacterium that causes both community- and healthcare-associated infections. Although various virulence factors and highly pathogenic phenotypes have been reported, the pathogenicity of K. pneumoniae is still not fully understood. In this study, we utilized whole-genome sequencing data of 168 clinical K. pneumoniae strains to assess pathogenicity. This work was based on the concept that the genetic composition of individual genomes (referred to as holistic gene content) of the strains may contribute to their pathogenicity. Holistic gene content analysis revealed two distinct groups of K. pneumoniae strains ('major group' and 'minor group'). The minor group included strains with known highly pathogenic clones (ST23, ST375, ST65 and ST86). The minor group had higher rates of capsular genotype K1 and presence of nine specific virulence genes (rmpA, iucA, iutA, irp2, fyuA, ybtS, iroN, allS and clbA) compared to the major group. Pathogenicity was assessed using Galleria mellonella larvae. Infection experiments revealed lower survival rates of larvae infected with strains from the minor group, indicating higher virulence. In addition, the minor group had a higher string test positivity rate than the major group. Holistic gene content analysis predicted possession of virulence genes, string test positivity and pathogenicity as observed in the G. mellonella infection model. Moreover, the findings suggested the presence of as yet unrecognized genomic elements that are either involved in the acquisition of virulence genes or associated with pathogenicity.

通过整体基因含量分析评估肺炎克雷伯氏菌的致病性。
肺炎克雷伯菌是一种革兰氏阴性菌,可引起社区和医疗保健相关感染。虽然已有各种毒力因子和高致病性表型的报道,但对肺炎克雷伯菌的致病性仍不完全清楚。在本研究中,我们利用 168 株临床肺炎克氏菌的全基因组测序数据来评估其致病性。这项工作基于这样一个概念,即菌株单个基因组的遗传组成(称为整体基因含量)可能会导致其致病性。整体基因含量分析显示,肺炎克雷伯菌株分为两个不同的群体("主要群体 "和 "次要群体")。次要组包括已知的高致病性克隆菌株(ST23、ST375、ST65 和 ST86)。与主要菌株组相比,次要菌株组的荚膜基因型 K1 和九种特定毒力基因(rmpA、iucA、iutA、irp2、fyuA、ybtS、iroN、allS 和 clbA)的出现率更高。致病性是用 Galleria mellonella 幼虫进行评估的。感染实验表明,感染次要组菌株的幼虫存活率较低,这表明其毒力较强。此外,次要组的串联检测阳性率高于主要组。通过整体基因含量分析,可以预测是否拥有毒力基因、串测阳性率和致病性,正如在 G. mellonella 感染模型中观察到的那样。此外,研究结果表明,存在一些尚未认识到的基因组元素,它们要么参与了毒力基因的获得,要么与致病性有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Microbial Genomics
Microbial Genomics Medicine-Epidemiology
CiteScore
6.60
自引率
2.60%
发文量
153
审稿时长
12 weeks
期刊介绍: Microbial Genomics (MGen) is a fully open access, mandatory open data and peer-reviewed journal publishing high-profile original research on archaea, bacteria, microbial eukaryotes and viruses.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信