Relationship of Mitochondrial DNA Oxidation and Content with Metabolic Syndrome and Cardiovascular Risk in Obesity Phenotypes.

IF 3.8 Q2 ENDOCRINOLOGY & METABOLISM

Journal of Obesity Pub Date : 2024-09-11 eCollection Date: 2024-01-01 DOI:10.1155/2024/3008093

Mailén Rojo, Hernán Pérez, Andrea Liliana Millán, María Constanza Pautasso, Gustavo Daniel Frechtel, Gloria Edith Cerrone

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Abstract

Objective: Obesity, chronic inflammation, and oxidative stress can influence mitochondrial DNA (mtDNA) content. Our objective was to evaluate the oxidation level and content of mtDNA and its relationship with metabolic parameters in metabolically healthy obese (MHO) compared to metabolically unhealthy obese (MUO) and normal weight (NW) controls.

Materials and methods: We studied 94 NW, 95 MHO, and 97 MUO individuals between 18 and 80 years old. Relative mtDNA content and mtDNA oxidation level (8-oxoguanine, 8-OxoG) were determined in peripheral blood leukocytes by the SYBR Green method of real-time PCR. One-way ANOVA and Tukey test were used to compare biochemical, clinical, and anthropometric characteristics, as well as mtDNA content and 8-OxoG.

Results: A progressive decrease in mtDNA content was observed between NW, MHO, and MUO with significant differences in MUO vs. NW (p: 0.04). An increase in 8-OxoG was observed in MUO patients compared to the other groups (MUO vs. MHO p: 0.01; MUO vs. NW p: 0.04). mtDNA content was directly correlated with HDL-c (p < 0.01) and inversely with waist circumference (p: 0.01) and LDL-c (p: 0.05). mtDNA content decreased, and the oxidation level increased concomitantly with the presence of obesity, the number of MS components, higher coronary risk, and insulin resistance parameters.

Conclusion: MHO presented a similar mtDNA oxidation level to NW and mtDNA content to the MUO, placing the MHO individuals as having an intermediate phenotype. Changes in mtDNA content and oxidation were correlated to the lipid profile related to obesity and/or MS presence, probably associated with oxidative stress and chronic low-grade inflammation.

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线粒体 DNA 氧化和含量与肥胖表型中代谢综合征和心血管风险的关系。

目的：肥胖、慢性炎症和氧化应激可影响线粒体 DNA（mtDNA）含量：肥胖、慢性炎症和氧化应激会影响线粒体 DNA（mtDNA）的含量。我们的目的是评估代谢健康肥胖（MHO）与代谢不健康肥胖（MUO）和正常体重（NW）对照组相比，线粒体 DNA 的氧化水平和含量及其与代谢参数的关系：我们研究了 94 名 NW、95 名 MHO 和 97 名 MUO，他们的年龄在 18 岁至 80 岁之间。采用 SYBR Green 实时 PCR 法测定外周血白细胞中相对 mtDNA 含量和 mtDNA 氧化水平（8-氧鸟嘌呤，8-OxoG）。采用单因素方差分析和 Tukey 检验比较生化、临床和人体测量特征以及 mtDNA 含量和 8-OxoG：结果：NW、MHO 和 MUO 之间的 mtDNA 含量呈逐渐下降趋势，MUO 与 NW 相比差异显著（p：0.04）。与其他组相比，在 MUO 患者中观察到 8-OxoG 增加（MUO vs. MHO p：0.01；MUO vs. NW p：0.04）。mtDNA 含量与 HDL-c 直接相关（p < 0.01），与腰围（p：0.mtDNA含量的降低和氧化水平的升高与肥胖的存在、MS成分的数量、较高的冠状动脉风险和胰岛素抵抗参数有关：MHO的mtDNA氧化水平与NW相似，mtDNA含量与MUO相似，因此MHO个体属于中间表型。mtDNA含量和氧化的变化与肥胖和/或多发性硬化症相关的血脂谱有关，可能与氧化应激和慢性低度炎症有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Obesity ENDOCRINOLOGY & METABOLISM-

CiteScore

7.50

自引率

3.00%

发文量

审稿时长

21 weeks

期刊介绍： Journal of Obesity is a peer-reviewed, Open Access journal that provides a multidisciplinary forum for basic and clinical research as well as applied studies in the areas of adipocyte biology & physiology, lipid metabolism, metabolic syndrome, diabetes, paediatric obesity, genetics, behavioural epidemiology, nutrition & eating disorders, exercise & human physiology, weight control and health risks associated with obesity.