Integrated Analysis of Noncoding RNAs (PVT-1 and miR-200c) and Their Correlation with STAT4/IL-6 Axis as Reliable Biomarkers for COVID-19 Severity.

IF 1.9 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Journal of Interferon and Cytokine Research Pub Date : 2024-11-01 Epub Date: 2024-09-20 DOI:10.1089/jir.2024.0132
Ghada Ayeldeen, Bahaa Mohammed Badr, Mohamed R Herzalla, Eman Amer, Mahmoud Elsabahy, Olfat G Shaker, Nabil A Hasona
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引用次数: 0

Abstract

Inefficient control of elevated inflammatory mediators in coronavirus disease 2019 (COVID-19) has led to health complications, prompting the exploration of efficient biomarkers for monitoring this condition. We herein sought to investigate the implications of plasmacytoma variant translocation 1 (PVT-1), microRNA-200c (miR-200c), signal transducer and activator of transcription 4 (STAT-4), and interleukin-6 (IL-6), as well as how they correlated with creatinine, C-reactive protein (CRP), and lactate dehydrogenase (LDH) activity to identify biomarkers able to the early prognosis and diagnosis of COVID-19. Our study included a total of 105 infected COVID-19 patients and 35 healthy subjects as controls. Individuals with COVID-19 showed a significant increase in CRP, creatinine, and LDH activity. In addition, COVID-19 patients exhibited significantly higher levels of IL-6. These patients also demonstrated notably elevated expressions of miR-200c and PVT-1. The expression level of STAT4 decreased in the COVID-19 patients, and this decrease was negatively correlated with creatinine and LDH activity. The levels of miR-200c and PVT-1 expressions, and their connections with IL-6 and STAT4 levels, increased significantly with the severity of COVID-19 cases. In addition, receiver operating characteristic analysis showed that PVT-1 and miR-200c could be reliable biomarkers for determining the severity of COVID-19.

综合分析非编码 RNA(PVT-1 和 miR-200c)及其与 STAT4/IL-6 轴的相关性,作为 COVID-19 严重程度的可靠生物标志物。
2019年冠状病毒病(COVID-19)中炎症介质的升高控制不力导致了健康并发症,这促使人们探索监测这种情况的有效生物标志物。在此,我们试图研究浆细胞瘤变异易位1(PVT-1)、microRNA-200c(miR-200c)、信号转导及激活转录4(STAT-4)和白细胞介素-6(IL-6)的影响,以及它们与肌酐、C反应蛋白(CRP)和乳酸脱氢酶(LDH)活性的相关性,以确定能够早期预后和诊断COVID-19的生物标志物。我们的研究共纳入了 105 名 COVID-19 感染者和 35 名健康对照者。COVID-19患者的 CRP、肌酐和 LDH 活性显著升高。此外,COVID-19 患者的 IL-6 水平明显升高。这些患者的 miR-200c 和 PVT-1 表达也明显升高。COVID-19 患者 STAT4 的表达水平下降,这种下降与肌酐和 LDH 活性呈负相关。miR-200c和PVT-1的表达水平及其与IL-6和STAT4水平的关系随着COVID-19病例的严重程度而显著增加。此外,接收器操作特征分析表明,PVT-1和miR-200c是判断COVID-19严重程度的可靠生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.80
自引率
0.00%
发文量
78
审稿时长
2.2 months
期刊介绍: Journal of Interferon & Cytokine Research (JICR) provides the latest groundbreaking research on all aspects of IFNs and cytokines. The Journal delivers current findings on emerging topics in this niche community, including the role of IFNs in the therapy of diseases such as multiple sclerosis, the understanding of the third class of IFNs, and the identification and function of IFN-inducible genes.
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