Melatonin protects against defects induced by methoxychlor in porcine oocyte maturation.

IF 3.4 3区 生物学 Q3 CELL BIOLOGY
Zhaojun Geng, Mingjun Zhang, Shuming Shi, Bing Hu, Liying Liu, Zhichao Chi, Linyi Qu, Yongxun Jin, Xianfeng Yu
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Abstract

Methoxychlor (MXC) is a widely used organochlorine pesticide primarily targeting pests. However, MXC has been found to negatively impact the reproductive system of both humans and animals, triggering oxidative stress and apoptosis. Melatonin (MLT), an endogenous hormone, possesses various benefits, including circadian rhythm regulation and anti-inflammatory and antioxidative stress effects. Moreover, MLT plays a crucial role in the development of animal germ cells and embryos. This study aimed to investigate the impact of MLT on porcine oocytes exposed to MXC. The experimental findings revealed that 200 μM MXC had detrimental effects on the maturation of porcine oocytes. However, the addition of 10-8 M MLT mitigated the toxic effects of MXC. MXC induced oxidative stress in porcine oocytes, leading to an increase in reactive oxygen species and impairing mitochondrial function. Consequently, oocyte quality was affected, resulting in elevated levels of early apoptosis and DNA damage, ultimately negatively impacting subsequent embryonic development. However, the addition of MLT showed the potential to ameliorate the damage caused by MXC. In conclusion, our results suggest that MLT exhibits a protective effect against MXC-induced damage to porcine oocyte maturation.

褪黑素可防止甲氧氯诱导的猪卵母细胞成熟缺陷。
甲氧氯(MXC)是一种广泛使用的有机氯杀虫剂,主要针对害虫。然而,研究发现甲氧氯会对人类和动物的生殖系统产生负面影响,引发氧化应激和细胞凋亡。褪黑素(MLT)是一种内源性激素,具有多种益处,包括昼夜节律调节、抗炎和抗氧化应激作用。此外,褪黑激素在动物生殖细胞和胚胎的发育过程中起着至关重要的作用。本研究旨在探讨 MLT 对暴露于 MXC 的猪卵母细胞的影响。实验结果表明,200 μM MXC 对猪卵母细胞的成熟有不利影响。然而,添加 10-8 MLT 可减轻 MXC 的毒性作用。MXC 在猪卵母细胞中诱导氧化应激,导致活性氧增加并损害线粒体功能。因此,卵母细胞质量受到影响,导致早期细胞凋亡和 DNA 损伤水平升高,最终对随后的胚胎发育产生负面影响。然而,添加 MLT 有可能改善 MXC 造成的损伤。总之,我们的研究结果表明,MLT 对 MXC 引起的猪卵母细胞成熟损伤具有保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell Cycle
Cell Cycle 生物-细胞生物学
CiteScore
7.70
自引率
2.30%
发文量
281
审稿时长
1 months
期刊介绍: Cell Cycle is a bi-weekly peer-reviewed journal of high priority research from all areas of cell biology. Cell Cycle covers all topics from yeast to man, from DNA to function, from development to aging, from stem cells to cell senescence, from metabolism to cell death, from cancer to neurobiology, from molecular biology to therapeutics. Our goal is fast publication of outstanding research.
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