Chemoenzymatic Synthesis of Keratan Sulfate Oligosaccharides Using UDP-Galactose-6-aldehyde To Control Sulfation at Galactosides.

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-10-04 Epub Date: 2024-09-23 DOI:10.1021/acs.orglett.4c02899

Yunfei Wu, Gerlof P Bosman, Gaël M Vos, Elif Uslu, Digantkumar Chapla, Chin Huang, Kelley W Moremen, Geert-Jan Boons

引用次数: 0

Abstract

Keratan sulfate (KS) is a highly complex proteoglycan that has a poly-LacNAc chain that can be modified by diverse patterns of sulfate esters at C-6 positions of galactoside (Gal) and N-acetylglucosamine (GlcNAc) residues. Here, a chemo-enzymatic methodology is described that can control the pattern of sulfation at Gal using UDP-Gal-aldehyde as a donor for poly-LacNAc assembly to temporarily block specific sites from sulfation by galactose 6-sulfotransferase (CHST1).

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利用 UDP-半乳糖-6-甲醛控制半乳糖苷硫酸化的化学酶法合成开拉坦硫酸酯低聚糖

硫酸角叉菜胶（KS）是一种高度复杂的蛋白多糖，其聚 LacNAc 链可通过半乳糖苷（Gal）和 N-乙酰葡糖胺（GlcNAc）残基 C-6 位上的硫酸酯的不同模式进行修饰。本文介绍了一种化学酶法，该方法可以利用 UDP-Gal-甲醛作为聚 LacNAc 组装的供体，暂时阻止特定位点被半乳糖 6-磺基转移酶（CHST1）硫酸化，从而控制 Gal 的硫酸化模式。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.