Efficient Plantlet Regeneration from Branches in Mangifera indica L.

Plants Pub Date : 2024-09-17 DOI:10.3390/plants13182595

Huijing Zhou, Jinglang Sun, Keyuan Zheng, Xinyuan Zhang, Yuan Yao, Mulan Zhu

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Abstract

Mango (Mangifera indica L.) is one of the most significant tropical and subtropical fruit species, with high ecological and economic value. However, research on the in vitro culture of mangoes is relatively weak, so establishing an efficient and stable mango plant regeneration system is of great significance. In this study, a preliminary mango regeneration system was established with Mangifera indica L. cv. Keitt from young branches as the starting explants. The results showed that the optimal plant growth regulator (PGR) formula for direct adventitious shoot induction on the branches was 1 mg/L 6-benzylaminopurine (6-BA) + 0.1 mg/L a-naphthaleneacetic acid (NAA), with an adventitious shoot induction rate of 73.63% and an average of 6.76 adventitious shoots. The optimal basal medium for adventitious shoot induction was wood plant medium (WPM), with an adventitious shoot induction rate of 63.87% and an average of 5.21 adventitious shoots. The optimal culture medium for adventitious shoot elongation was WPM + 1 mg/L 6-BA + 0.5 mg/L NAA, with an adventitious shoot elongation rate of 89.33% and an average length of 5.17 cm. The optimal formula for the induction of mango rooting was Douglas fir cotyledon revised medium (DCR) + 3 mg/L indole-3-butyric acid (IBA), with a maximum rooting rate of 66.13% and an average rooting quantity of 6.43. The genetic fidelity of the in vitro-regenerated plants was evaluated using inter-simple sequence repeat (ISSR) molecular markers. There was no difference between the in vitro-regenerated plants and the parent plant. This study provides an efficient and stable propagation system for Mangifera indica L., laying the foundation for its rapid propagation and genetic improvement.

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从 Mangifera indica L. 的分枝中高效再生小植株

芒果（Mangifera indica L.）是最重要的热带和亚热带水果品种之一，具有很高的生态和经济价值。然而，对芒果离体培养的研究相对薄弱，因此建立高效稳定的芒果植株再生系统意义重大。本研究以 Mangifera indica L. cv. Keitt 的幼枝为起始外植体，初步建立了芒果再生系统。结果表明，在枝条上直接诱导不定芽的最佳植物生长调节剂（PGR）配方为 1 mg/L 6-benzylaminopurine (6-BA) + 0.1 mg/L a-naphthaleneacetic acid (NAA)，其不定芽诱导率为 73.63%，平均诱导 6.76 个不定芽。诱导不定芽的最佳基础培养基是木质植物培养基（WPM），其不定芽诱导率为 63.87%，平均 5.21 个不定芽。不定芽伸长的最佳培养基为 WPM + 1 mg/L 6-BA + 0.5 mg/L NAA，不定芽伸长率为 89.33%，平均长度为 5.17 厘米。诱导芒果生根的最佳配方是花旗松子叶修订培养基（DCR）+ 3 mg/L 吲哚-3-丁酸（IBA），最高生根率为 66.13%，平均生根量为 6.43。利用简单序列重复（ISSR）分子标记对离体再生植株的遗传保真度进行了评估。结果表明，离体再生植株与亲本植株之间没有差异。这项研究为芒果提供了一个高效稳定的繁殖系统，为其快速繁殖和遗传改良奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Plants

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