Discovery of GJC1 as a prognostic biomarker in glioma cells: insights into its cell-cycle relationship and differential expression in non-neuronal cells

IF 4.2 3区 医学 Q2 NEUROSCIENCES
Xiangtian Ji, Xin Chen, Guozhong Lin, Kaiming Ma, Junhua Yang, Xiaofang Zhao, Suhua Chen, Jun Yang
{"title":"Discovery of GJC1 as a prognostic biomarker in glioma cells: insights into its cell-cycle relationship and differential expression in non-neuronal cells","authors":"Xiangtian Ji, Xin Chen, Guozhong Lin, Kaiming Ma, Junhua Yang, Xiaofang Zhao, Suhua Chen, Jun Yang","doi":"10.3389/fncel.2024.1440409","DOIUrl":null,"url":null,"abstract":"BackgroundGliomas, originating from the most common non-neuronal cells in the brain (glial cells), are the most common brain tumors and are associated with high mortality and poor prognosis. Glioma cells exhibit a tendency to disrupt normal cell-cycle regulation, leading to abnormal proliferation and malignant growth. This study investigated the predictive potential of <jats:italic>GJC1</jats:italic> in gliomas and explored its relationship with the cell cycle.MethodsRetrospective analysis of RNA-seq and single-cell sequencing data was conducted using the Chinese Glioma Genome Atlas (CGGA) and The Cancer Genome Atlas (TCGA) databases. The differential expression of <jats:italic>GJC1</jats:italic> in gliomas with various pathological features and in different non-neuronal cell groups was analyzed. Functional data were examined using gene set variation analysis (GSVA). Furthermore, CellMiner was used to evaluate the relationship between <jats:italic>GJC1</jats:italic> expression and predicted treatment response across these databases.Results<jats:italic>GJC1</jats:italic> expression was enriched in high-grade gliomas and 1p/19q non-codeletion gliomas. <jats:italic>GJC1</jats:italic> enrichment was observed in classical and mesenchymal subtypes within the TCGA glioma subtype group. In single-cell subgroup analysis, <jats:italic>GJC1</jats:italic> expression was higher in glioma tissues compared to other non-neuronal cells. Additionally, the TCGA classical subtype of glioma cells exhibited more <jats:italic>GJC1</jats:italic> expression than the other subgroups. <jats:italic>GJC1</jats:italic> emerged as an independent prognostic factor for overall survival in glioma. GSVA unveiled potential mechanisms by which <jats:italic>GJC1</jats:italic> may impact cell-cycle regulation in glioma. Finally, a significant correlation was observed between <jats:italic>GJC1</jats:italic> expression and the sensitivity of multiple anti-cancer drugs.ConclusionThese findings confirmed <jats:italic>GJC1</jats:italic> as a novel biomarker and provided insights into the differential gene expression in non-neuronal cells and the impact of the cell cycle on gliomas. Consequently, <jats:italic>GJC1</jats:italic> may be used to predict glioma prognosis and has potential therapeutic value.","PeriodicalId":12432,"journal":{"name":"Frontiers in Cellular Neuroscience","volume":"39 1","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Cellular Neuroscience","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fncel.2024.1440409","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

BackgroundGliomas, originating from the most common non-neuronal cells in the brain (glial cells), are the most common brain tumors and are associated with high mortality and poor prognosis. Glioma cells exhibit a tendency to disrupt normal cell-cycle regulation, leading to abnormal proliferation and malignant growth. This study investigated the predictive potential of GJC1 in gliomas and explored its relationship with the cell cycle.MethodsRetrospective analysis of RNA-seq and single-cell sequencing data was conducted using the Chinese Glioma Genome Atlas (CGGA) and The Cancer Genome Atlas (TCGA) databases. The differential expression of GJC1 in gliomas with various pathological features and in different non-neuronal cell groups was analyzed. Functional data were examined using gene set variation analysis (GSVA). Furthermore, CellMiner was used to evaluate the relationship between GJC1 expression and predicted treatment response across these databases.ResultsGJC1 expression was enriched in high-grade gliomas and 1p/19q non-codeletion gliomas. GJC1 enrichment was observed in classical and mesenchymal subtypes within the TCGA glioma subtype group. In single-cell subgroup analysis, GJC1 expression was higher in glioma tissues compared to other non-neuronal cells. Additionally, the TCGA classical subtype of glioma cells exhibited more GJC1 expression than the other subgroups. GJC1 emerged as an independent prognostic factor for overall survival in glioma. GSVA unveiled potential mechanisms by which GJC1 may impact cell-cycle regulation in glioma. Finally, a significant correlation was observed between GJC1 expression and the sensitivity of multiple anti-cancer drugs.ConclusionThese findings confirmed GJC1 as a novel biomarker and provided insights into the differential gene expression in non-neuronal cells and the impact of the cell cycle on gliomas. Consequently, GJC1 may be used to predict glioma prognosis and has potential therapeutic value.
发现作为胶质瘤细胞预后生物标志物的 GJC1:深入了解其细胞周期关系和在非神经元细胞中的差异表达
背景胶质瘤起源于大脑中最常见的非神经元细胞(胶质细胞),是最常见的脑肿瘤,死亡率高,预后差。胶质瘤细胞有破坏正常细胞周期调节的倾向,导致异常增殖和恶性生长。本研究调查了GJC1在胶质瘤中的预测潜力,并探讨了其与细胞周期的关系。方法利用中国胶质瘤基因组图谱(CGGA)和癌症基因组图谱(TCGA)数据库对RNA-seq和单细胞测序数据进行了回顾性分析。分析了 GJC1 在具有不同病理特征的胶质瘤和不同非神经细胞组中的差异表达。使用基因组变异分析(GSVA)检验了功能数据。结果GJC1的表达在高级别胶质瘤和1p/19q非编码缺失胶质瘤中富集。在TCGA胶质瘤亚型组中的经典亚型和间质亚型中也观察到了GJC1的富集。在单细胞亚组分析中,GJC1在胶质瘤组织中的表达高于其他非神经元细胞。此外,TCGA 经典亚型胶质瘤细胞的 GJC1 表达高于其他亚组。GJC1成为胶质瘤总生存期的独立预后因素。GSVA揭示了GJC1可能影响胶质瘤细胞周期调控的潜在机制。结论这些发现证实了 GJC1 是一种新型生物标记物,并为非神经元细胞中不同基因的表达以及细胞周期对胶质瘤的影响提供了见解。因此,GJC1 可用于预测胶质瘤的预后,并具有潜在的治疗价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
7.90
自引率
3.80%
发文量
627
审稿时长
6-12 weeks
期刊介绍: Frontiers in Cellular Neuroscience is a leading journal in its field, publishing rigorously peer-reviewed research that advances our understanding of the cellular mechanisms underlying cell function in the nervous system across all species. Specialty Chief Editors Egidio D‘Angelo at the University of Pavia and Christian Hansel at the University of Chicago are supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信