Investigating the Impact of Hydrophobic Deep Eutectic Oil-in-Water Nanoemulsion on Cell Membrane Degradation and Inhibition of C. gloeosporioides in Postharvest Technology

IF 5.3 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food and Bioprocess Technology Pub Date : 2024-09-16 DOI:10.1007/s11947-024-03587-7

Gidado M. J., Ahmad Anas Nagoor Gunny, Subash C. B. Gopinath, Noor Hasyierah Mohd Salleh, Sunil Pareek, Kunasundari Balakrishnan

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Abstract

This study examines the effects of a hydrophobic deep eutectic oil-in-water nanoemulsion (HyDEN) on the cell membrane degradation and inhibition of Colletotrichum gloeosporioides. By analyzing post-treatment cell membrane morphology, we gain insights into the efficacy and mechanisms of action of antifungal agents. Significant changes such as disruption, collapse, wrinkling, and lysis were observed in the external morphology of C. gloeosporioides treated with HyDEN. When comparing control and treatment groups, HyDEN demonstrated more pronounced disruption and greater mycelial growth inhibition than Globus 5.5. HyDEN also effectively inhibited spore germination compared to Globus 5.5. Tests on intracellular ion leakage showed that HyDEN caused higher conductivity, indicating significant membrane disruption. Additionally, HyDEN led to a greater release of soluble sugars due to membrane damage compared to Globus 5.5. These findings suggest that HyDEN is a promising antifungal strategy, effectively disrupting cell wall and membrane functionality, and inhibiting fungal growth.

Graphical Abstract

Abstract Image

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研究疏水性深共晶水包油型纳米乳液对收获后技术中细胞膜降解和球孢子菌抑制的影响

本研究探讨了疏水性深共晶水包油型纳米乳液（HyDEN）对细胞膜降解和抑制球孢子菌（Colletotrichum gloeosporioides）的影响。通过分析处理后的细胞膜形态，我们可以深入了解抗真菌剂的功效和作用机制。经 HyDEN 处理的球孢子菌的外部形态发生了显著变化，如破坏、塌陷、起皱和裂解。在比较对照组和处理组时，HyDEN 比 Globus 5.5 显示出更明显的破坏作用和更强的菌丝生长抑制作用。与 Globus 5.5 相比，HyDEN 还能有效抑制孢子萌发。对细胞内离子泄漏的测试表明，HyDEN 会导致更高的传导性，这表明膜受到了严重破坏。此外，与 Globus 5.5 相比，HyDEN 还会因膜破坏而导致更多可溶性糖的释放。这些研究结果表明，HyDEN 是一种很有前途的抗真菌策略，它能有效破坏细胞壁和细胞膜的功能，抑制真菌生长。

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来源期刊

Food and Bioprocess Technology 农林科学-食品科技

CiteScore

9.50

自引率

19.60%

发文量

200

审稿时长

2.8 months

期刊介绍： Food and Bioprocess Technology provides an effective and timely platform for cutting-edge high quality original papers in the engineering and science of all types of food processing technologies, from the original food supply source to the consumer’s dinner table. It aims to be a leading international journal for the multidisciplinary agri-food research community. The journal focuses especially on experimental or theoretical research findings that have the potential for helping the agri-food industry to improve process efficiency, enhance product quality and, extend shelf-life of fresh and processed agri-food products. The editors present critical reviews on new perspectives to established processes, innovative and emerging technologies, and trends and future research in food and bioproducts processing. The journal also publishes short communications for rapidly disseminating preliminary results, letters to the Editor on recent developments and controversy, and book reviews.